eprintid: 5541
rev_number: 7
eprint_status: archive
userid: 30
dir: disk0/00/00/55/41
datestamp: 2012-02-18 09:34:40
lastmod: 2012-02-18 09:34:40
status_changed: 2012-02-18 09:34:40
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Gopalakrishnan, S
creators_name: Beale, M H
creators_name: Ward, J L
creators_name: Strange, R N
icrisatcreators_name: Gopalakrishnan, S
affiliation: University College London(London)
affiliation: The National Centre for Plant and Microbial Metabolomics(Harpenden)
affiliation: ICRISAT(Patancheru)
country: UK
country: India
title: Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum
ispublished: pub
subjects: s1.1
full_text_status: restricted
note: We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant “Ascorab” (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry
abstract: Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.





date: 2005
date_type: published
publication: Phytochemistry
volume: 66
number: 13
publisher: Elsevier
pagerange: 1536-1539
refereed: TRUE
issn: 0031-9422
official_url: http://dx.doi.org/10.1016/j.phytochem.2005.04.037
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22Chickpea+wilt%3A+identification+and+toxicity+of+8-O-methyl-fusarubin+from+Fusarium+acutatum%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
funders: European Union
citation:   Gopalakrishnan, S and Beale, M H and Ward, J L and Strange, R N  (2005) Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum.  Phytochemistry, 66 (13).  pp. 1536-1539.  ISSN 0031-9422     
document_url: http://oar.icrisat.org/5541/1/Phytochemistry_66_1536%E2%80%931539_2005.pdf