%O We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant “Ascorab” (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry
%A S Gopalakrishnan
%A M H Beale
%A J L Ward
%A R N Strange
%I Elsevier
%V 66
%L icrisat5541
%J Phytochemistry
%N 13
%P 1536-1539
%D 2005
%X Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.





%T Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum