TY  - JOUR
AV  - restricted
A1  - Gopalakrishnan, S
A1  - Beale, M H
A1  - Ward, J L
A1  - Strange, R N
TI  - Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum
UR  - http://dx.doi.org/10.1016/j.phytochem.2005.04.037
JF  - Phytochemistry
SN  - 0031-9422
PB  - Elsevier
N1  - We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant ?Ascorab? (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry
N2  - Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and ?max at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.





Y1  - 2005///
SP  - 1536
ID  - icrisat5541
EP  - 1539
VL  - 66
IS  - 13
ER  -