http://oar.icrisat.org/id/eprint/5541 2012-02-18T09:34:40Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/5541/ Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum Gopalakrishnan, S Beale, M H Ward, J L Strange, R N Chickpea Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml. Elsevier 2005 Article PeerReviewed application/pdf en http://oar.icrisat.org/5541/1/Phytochemistry_66_1536%E2%80%931539_2005.pdf Gopalakrishnan, S and Beale, M H and Ward, J L and Strange, R N (2005) Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum. Phytochemistry, 66 (13). pp. 1536-1539. ISSN 0031-9422 http://dx.doi.org/10.1016/j.phytochem.2005.04.037 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage