relation: http://oar.icrisat.org/5541/
title: Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum
creator: Gopalakrishnan, S
creator: Beale, M H
creator: Ward, J L
creator: Strange, R N
subject: Chickpea
description: Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.          
publisher: Elsevier
date: 2005
type: Article
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/5541/1/Phytochemistry_66_1536%E2%80%931539_2005.pdf
identifier:   Gopalakrishnan, S and Beale, M H and Ward, J L and Strange, R N  (2005) Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum.  Phytochemistry, 66 (13).  pp. 1536-1539.  ISSN 0031-9422     
relation: http://dx.doi.org/10.1016/j.phytochem.2005.04.037