"5541","7","archive","30",,,"disk0/00/00/55/41","2012-02-18 09:34:40","2012-02-18 09:34:40","2012-02-18 09:34:40","article",,,"show","Library-ICRISAT@cgiar.org",,,"","","","","","","","","","",,,,"Gopalakrishnan","S","","","","","","","Gopalakrishnan","S",,,"","",,,,,"","",,,,,"","","University College London(London)","UK","Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum","pub","s1.1","","","restricted",,,,,"We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant “Ascorab” (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry","Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.




","2005","published",,"Phytochemistry","66","13","Elsevier",,"1536-1539",,,,,,,,,,,"TRUE",,"0031-9422",,,,,,"","http://dx.doi.org/10.1016/j.phytochem.2005.04.037","http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22Chickpea+wilt%3A+identification+and+toxicity+of+8-O-methyl-fusarubin+from+Fusarium+acutatum%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0","author",,"European Union","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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