@article{icrisat5541,
           title = {Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum},
       publisher = {Elsevier},
            year = {2005},
          author = {S Gopalakrishnan and M H Beale and J L Ward and R N Strange},
           pages = {1536--1539},
          volume = {66},
            note = {We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant ?Ascorab? (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry},
         journal = {Phytochemistry},
          number = {13},
             url = {http://oar.icrisat.org/5541/},
        abstract = {Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and {\ensuremath{\lambda}}max at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.




}
}