<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops"^^ . "Molecular markers and genetic maps are the pre‐requisites for trait mapping and genomics‐assisted crop\r\nimprovement. However, very limited genomic resources were available until recently for the legume crops important\r\nin the semi‐arid tropics (SAT). As a part of several initiatives, species‐specific genomic resources are now being\r\ndeveloped in most of these legume crops. For instance, using simple sequence repeat (SSR)‐enriched libraries and\r\nbacterial artificial chromosome (BAC)‐end sequence mining approaches, nearly 1,500‐ 3,000 novel SSR markers\r\nhave been developed for chickpea, pigeonpea and groundnut. In addition, next generation sequencing technologies\r\nlike Roche 454/FLX and Illumina/Solexa, in addition to Sanger sequencing, are being used to sequence the\r\ntranscriptomes of reference or parental genotypes of mapping populations of chickpea and pigeonpea to access the\r\ngene space and develop functional markers. Based on Sanger and 454/FLX transcript reads, transcriptome\r\nassemblies have been developed for chickpea (103,215 tentative unique sequences, TUSs) and pigeonpea (127,754\r\nTUSs) that are being characterized using genome sequence data of Medicago and soybean. In parallel, RNA of four\r\nchickpea and twelve pigeonpea genotypes, that represent parents of different mapping populations, have been\r\nsequenced by using Illumina/Solexa sequencing approach that has resulted ca. 120 million reads for chickpea and 20\r\nmillion reads for pigeonpea. Alignment of these Illumina/Solexa reads of these genotypes with transcriptome\r\nassembly of the respective species has provided a large number (tens of thousands) of SNPs. Selected set of SNPs are\r\nbeing used to develop large‐scale SNP genotyping platform in chickpea and pigeonpea. By using the existing\r\nresource of SSR, SNP and DArT markers, high‐density genetic maps are being developed in these species for trait\r\nmapping and molecular breeding. It is anticipated that molecular breeding practice may be routine and part of\r\nbreeding activities in the SAT legumes in coming future"^^ . "2011" . . . . . . . . "R K"^^ . "Varshney"^^ . "R K Varshney"^^ . . . . "National Symposium on Genomics and Crop Improvement: Relevance and Reservations "^^ . . . . . "Acharya N G Ranga Agricultural University, Rajendranagar, Hyd"^^ . . . . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (PDF)"^^ . . . . . "GenomicsandCropImprovementRelevanceandReservations_2010.pdf"^^ . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (Indexer Terms)"^^ . . . . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (Image (PNG))"^^ . . . . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (Image (PNG))"^^ . . . . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (Image (PNG))"^^ . . . . . . "Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops (Image (PNG))"^^ . . . . . "HTML Summary of #5489 \n\nApplication of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops\n\n" . "text/html" . . . "Chickpea"@en . . . "Pigeonpea"@en . . . "Groundnut"@en . .