eprintid: 5142 rev_number: 8 eprint_status: archive userid: 14 dir: disk0/00/00/51/42 datestamp: 2011-12-28 03:18:36 lastmod: 2011-12-28 03:18:36 status_changed: 2011-12-28 03:18:36 type: conference_item metadata_visibility: show contact_email: Library-ICRISAT@cgiar.org creators_name: Nolt, B L creators_name: Rajeshwari, R creators_name: Bharathan, N creators_name: Reddy, D V R icrisatcreators_name: Nolt, B L icrisatcreators_name: Rajeshwari, R icrisatcreators_name: Bharathan, N icrisatcreators_name: Reddy, D V R affiliation: ICRISAT(Patancheru) country: India title: Improved Serological Techniques for the Detection and Identification of Groundnut Viruses ispublished: pub subjects: s1.3 full_text_status: public pres_type: paper abstract: Serology is indispensable for the detection and identification of plant viruses. Recently, the highly sensitive enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) techniques have been developed and may replace some of the conventional serological methods. The direct or standard double-antibody sandwich (DAS) form of ELISA. as first described by Clark and Adams (1977), has wide applications in plant virology. An indirect form of ELISA (I-ELISA) has recently been developed by Barbara and Clark (1982). The ISEM procedure developed by Derrick (1973) combines the specificity of serology with coventional electron microscopy. DAS-ELISA, I-ELISA and ISEM have been adapted for the detection and identification of several groundnut viruses. This paper will provide a description of each technique and its application for the detection and characterization of viruses occurring on groundnut in India. date: 1983 date_type: published publication: Management of Diseases of Oilseed Crops pagerange: 1-6 event_title: Proceedings of the National Seminar on Management of Diseases of Oilseed Crops event_location: Madurai event_dates: 21st - 22nd January 1983 event_type: conference refereed: TRUE related_url_url: http://scholar.google.co.in/scholar?as_q=Improved+Serological+Techniques+for+the+Detection+and+Identification+of+Groundnut+Viruses&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_sdt related_url_type: author citation: Nolt, B L and Rajeshwari, R and Bharathan, N and Reddy, D V R (1983) Improved Serological Techniques for the Detection and Identification of Groundnut Viruses. In: Proceedings of the National Seminar on Management of Diseases of Oilseed Crops, 21st - 22nd January 1983, Madurai. document_url: http://oar.icrisat.org/5142/1/0125.pdf