OAR@ICRISATImproved Serological Techniques for the Detection and Identification of Groundnut VirusesB LNoltauthorRRajeshwariauthorNBharathanauthorD V RReddyauthorSerology is indispensable for the detection and identification of plant viruses. Recently, the highly sensitive enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) techniques have been developed and may replace some of the conventional serological methods. The direct or standard double-antibody sandwich (DAS) form of ELISA. as first described by Clark and Adams (1977), has wide applications in plant virology. An indirect form of ELISA (I-ELISA) has recently been developed by Barbara and Clark (1982). The ISEM procedure developed by Derrick (1973) combines the specificity of serology with coventional electron microscopy. DAS-ELISA, I-ELISA and ISEM have been adapted for the detection and identification of several groundnut viruses. This paper will provide a description of each technique and its application for the detection and characterization of viruses occurring on groundnut in India.Groundnut1983Conference or Workshop Item

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