http://oar.icrisat.org/id/eprint/5142 2011-12-28T03:18:36Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/5142/ Improved Serological Techniques for the Detection and Identification of Groundnut Viruses Nolt, B L Rajeshwari, R Bharathan, N Reddy, D V R Groundnut Serology is indispensable for the detection and identification of plant viruses. Recently, the highly sensitive enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) techniques have been developed and may replace some of the conventional serological methods. The direct or standard double-antibody sandwich (DAS) form of ELISA. as first described by Clark and Adams (1977), has wide applications in plant virology. An indirect form of ELISA (I-ELISA) has recently been developed by Barbara and Clark (1982). The ISEM procedure developed by Derrick (1973) combines the specificity of serology with coventional electron microscopy. DAS-ELISA, I-ELISA and ISEM have been adapted for the detection and identification of several groundnut viruses. This paper will provide a description of each technique and its application for the detection and characterization of viruses occurring on groundnut in India. 1983 Conference or Workshop Item PeerReviewed application/pdf en http://oar.icrisat.org/5142/1/0125.pdf Nolt, B L and Rajeshwari, R and Bharathan, N and Reddy, D V R (1983) Improved Serological Techniques for the Detection and Identification of Groundnut Viruses. In: Proceedings of the National Seminar on Management of Diseases of Oilseed Crops, 21st - 22nd January 1983, Madurai. info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage