@inproceedings{icrisat5142,
       booktitle = {Proceedings of the National Seminar on Management of Diseases of Oilseed Crops},
            year = {1983},
           title = {Improved Serological Techniques for the Detection and Identification of Groundnut Viruses},
           pages = {1--6},
          author = {B L Nolt and R Rajeshwari and N Bharathan and D V R Reddy},
         journal = {Management of Diseases of Oilseed Crops},
             url = {http://oar.icrisat.org/5142/},
        abstract = {Serology is indispensable for the detection
and identification of plant viruses.
Recently, the highly sensitive enzyme-linked
immunosorbent assay (ELISA) and immunosorbent
electron microscopy (ISEM) techniques
have been developed and may replace
some of the conventional serological methods.
The direct or standard double-antibody
sandwich (DAS) form of ELISA. as first
described by Clark and Adams (1977), has
wide applications in plant virology. An
indirect form of ELISA (I-ELISA) has recently
been developed by Barbara and Clark
(1982). The ISEM procedure developed by
Derrick (1973) combines the specificity of
serology with coventional electron microscopy.
DAS-ELISA, I-ELISA and ISEM have
been adapted for the detection and identification
of several groundnut viruses. This paper
will provide a description of each technique
and its application for the detection and characterization
of viruses occurring on groundnut
in India.}
}