TY  - JOUR
N2  - A protocol for plant regeneration from
mesophyll/protoplasts of sorghum [Sorghum bicolor
(L.) Moench] was developed. The yield of intact protoplasts,
their subsequent divisions and regeneration
were genotype-dependent. The genotype 296B was
always more responsive than IS 32266. For 296B, the
sixth leaf from 18-day-old plants kept in dark for 2 days
before harvesting was found to be the most suitable
source of viable protoplasts. The first division was
observed 10?12 days after plating, and the second division
after 12?14 days. The maximum plating efficiency
was 4.8% in 296 B, followed by 2.48% in IS 32266.
Microcolonies were visible after 25?30 days, and microcalli
after 60?75 days. Whole plants were obtained after
6?8 weeks of culture of microcalli on MS medium
containing 0.2 mg l?1 kinetin and 2 mg l?1 BAP. The
frequency of regeneration in 296B and IS 32266 was
12.80% and 10.58%, respectively. Ten plants transferred
to pots in the glasshouse established well. The
seeds collected from glasshouse-grown plants were
sown in the field where plants were grown to
maturity.
AV  - restricted
A1  - Sairam, R V
A1  - Seetharama, N
A1  - Devi, P S
A1  - Verma, A
A1  - Murthy, U R
TI  - Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]
UR  - http://dx.doi.org/10.1007/s002990050693
JF  - Plant Cell Reports
SP  - 972
Y1  - 1999///
ID  - icrisat5113
EP  - 977
SN  - 0721-7714
VL  - 18
PB  - Springer Verlag
IS  - 12
ER  -