<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]"^^ . "A protocol for plant regeneration from\r\nmesophyll/protoplasts of sorghum [Sorghum bicolor\r\n(L.) Moench] was developed. The yield of intact protoplasts,\r\ntheir subsequent divisions and regeneration\r\nwere genotype-dependent. The genotype 296B was\r\nalways more responsive than IS 32266. For 296B, the\r\nsixth leaf from 18-day-old plants kept in dark for 2 days\r\nbefore harvesting was found to be the most suitable\r\nsource of viable protoplasts. The first division was\r\nobserved 10–12 days after plating, and the second division\r\nafter 12–14 days. The maximum plating efficiency\r\nwas 4.8% in 296 B, followed by 2.48% in IS 32266.\r\nMicrocolonies were visible after 25–30 days, and microcalli\r\nafter 60–75 days. Whole plants were obtained after\r\n6–8 weeks of culture of microcalli on MS medium\r\ncontaining 0.2 mg l–1 kinetin and 2 mg l–1 BAP. The\r\nfrequency of regeneration in 296B and IS 32266 was\r\n12.80% and 10.58%, respectively. Ten plants transferred\r\nto pots in the glasshouse established well. The\r\nseeds collected from glasshouse-grown plants were\r\nsown in the field where plants were grown to\r\nmaturity."^^ . "1999" . . "18" . "12" . . "Springer Verlag"^^ . . . "Plant Cell Reports"^^ . . . "07217714" . . . . . . . . . . . . . . . . . . . "A"^^ . "Verma"^^ . "A Verma"^^ . . "R V"^^ . "Sairam"^^ . "R V Sairam"^^ . . "P S"^^ . "Devi"^^ . "P S Devi"^^ . . "U R"^^ . "Murthy"^^ . "U R Murthy"^^ . . "N"^^ . "Seetharama"^^ . "N Seetharama"^^ . . . . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (PDF)"^^ . . . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (Image (PNG))"^^ . . . . . . "lightbox.jpg"^^ . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (Image (PNG))"^^ . . . . . . "preview.jpg"^^ . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (Image (PNG))"^^ . . . . . . "medium.jpg"^^ . . . "Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench] (Image (PNG))"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #5113 \n\nCulture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]\n\n" . "text/html" . . . "Sorghum"@en . .