eprintid: 5067
rev_number: 9
eprint_status: archive
userid: 14
dir: disk0/00/00/50/67
datestamp: 2011-12-28 10:55:39
lastmod: 2011-12-28 10:55:39
status_changed: 2011-12-28 10:55:39
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Devi, K T
creators_name: Miller, J S
creators_name: Reddy, G
creators_name: Reddy, D V R
creators_name: Mayo, M A
icrisatcreators_name: Devi, K T
icrisatcreators_name: Reddy, D V R
affiliation: ICRISAT(Patancheru)
affiliation: Scottish Crop Research Institute(Dundee)
affiliation: Osmania University(Hyderabad)
country: India
country: UK
title: Phage-displayed peptides that mimic aflatoxin B1 in serological reactivity
ispublished: pub
subjects: s2.4
full_text_status: restricted
note: This document is an output from a project (R7083) funded by the UK Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of DFID. The authors are grateful to Dr Angelika Zeigler and Michele Liney for helpful discussions during this work, and to Jim McNicol, BioSS, for statistical guidance. MAM acknowledges financial support from the Scottish Executive Rural Affairs Department. KT-D and DVRR are supported by ICRISAT.

abstract: Aims: To test phage-displayed random peptide libraries as sources of peptides that mimic the binding of aflatoxin B1 to monoclonal antibodies raised against the toxin.

Methods and Results: For two of the three MAbs tested, clones were obtained by panning, producing phage that bound specifically to MAb 13D1–1D9 (MAb 24; specific for aflatoxins B1 and G1) and MAb 6E12–1E9 (MAb 13; specific for aflatoxins B1, G1 and B2) in ELISA. The amino acid sequences of the binding peptides varied. Those binding to MAb 24 contained the sequence of ‘…YMD…’, and those that bound to MAb 13 contained the dipeptide ‘PW’. Mimotope phage was used in a competition ELISA format for assaying aflatoxin concentrations.

Conclusions: The results show that mimotope preparations are effective substitutes for pure toxin in these ELISA procedures.

Significance and Impact of the Study: These results should contribute significantly to enhancing the safety and diminishing the costs of aflatoxin assays.


date: 2001
date_type: published
publication: Journal of Applied Microbiology
volume: 90
number: 3
publisher: Willey-Blackwell
pagerange: 330-336
refereed: TRUE
issn: 1365-2672
official_url: http://dx.doi.org/10.1046/j.1365-2672.2001.01249.x
related_url_url: http://scholar.google.co.in/scholar?as_q=Phage-displayed+peptides+that+mimic+aflatoxin+B1+in+serological+reactivity+&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_sdtp=on&as_sdtf=&
related_url_type: author
funders: Department for International Development
funders: Scottish Executive Rural Affairs Department
funders: International Crops Research Institute for the Semi-Arid Tropics
citation:   Devi, K T and Miller, J S and Reddy, G and Reddy, D V R and Mayo, M A  (2001) Phage-displayed peptides that mimic aflatoxin B1 in serological reactivity.  Journal of Applied Microbiology, 90 (3).  pp. 330-336.  ISSN 1365-2672     
document_url: http://oar.icrisat.org/5067/1/JAppMicro90%283%29330-336.pdf