eprintid: 4937
rev_number: 8
eprint_status: archive
userid: 14
dir: disk0/00/00/49/37
datestamp: 2011-12-13 11:32:33
lastmod: 2011-12-13 11:32:33
status_changed: 2011-12-13 11:32:33
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
creators_name: Hazra, Shuvendu
creators_name: Thakur, R P
creators_name: Devi, G U
creators_name: Mathur, K
icrisatcreators_name: Thakur, R P
affiliation: ANGRAU(Hyderabad)
affiliation: ICRISAT(Patancheru)
affiliation: SK Rajasthan Agricultural University(Bikaner)
country: India
title: Pathogenic and Molecular Variability Among Twelve Isolates of Colletotrichum graminicola from Sorghum
ispublished: pub
subjects: s1.4
full_text_status: restricted
keywords: Sorghum bicolor, Colletotrichum graminicola, virulence, pathotypes, DNA polymorphism
note: The senior author is highly thankful to Dr. S. Sivaramakrishnan, Scientist (Biochemistry), ICRISAT for his guidance to conduct molecular work and to Mr. K.D.V. Prasad for his help to prepare dendrograms.
abstract: Variability in 12 foliar and grain isolates of sorghum anthracnose pathogen Colletotrichum graminicola was studied through virulence and Random Amplified Polymorphic DNA ( RAPD) assay. For virulence analysis, pot grown 21-day-old plants of six differential sorghum lines were spray inoculated with conidia (1 x 105 conidia ml-1). The isolates showed significant (P< 0.05) variations in latent period, aggressiveness and virulence. Overall pathogenic potential was determined as Virulence Index. Hierarchical clustering using euclidean test based on "Virulence Index" grouped the 12 isolates into three groups, while that based on RAPDs using primer OPA8 grouped these into four groups. Monoconidial isolate Cg 226-3 had 85% genetic and pathogenic similarity like its parent, while Cg 227-3 showed virulence variation and 65% genetic similarity with its parent, indicating heterogeneity in Cg 227.
date: 1999
date_type: published
publication: Indian Journal of Mycology & Plant Pathology
volume: 29
number: 2
publisher: Indian Society of Mycology and Plant Pathology
pagerange: 176-183
refereed: TRUE
issn: 0303-4097
related_url_url: http://scholar.google.co.in/scholar?as_q=Pathogenic+and+Molecular+Variability+Among+Twelve+Isolates+of+Colletotrichum+graminicola+from+Sorghum&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_s
related_url_type: author
citation:   Hazra, Shuvendu and Thakur, R P and Devi, G U and Mathur, K  (1999) Pathogenic and Molecular Variability Among Twelve Isolates of Colletotrichum graminicola from Sorghum.  Indian Journal of Mycology & Plant Pathology, 29 (2).  pp. 176-183.  ISSN 0303-4097     
document_url: http://oar.icrisat.org/4937/1/Sg.Pathogenic%20%26%20molecular.1999.SH%2CRPT%2CGUD%2CKM.JMPP%20%2029%20176-183.pdf