TY - JOUR AV - restricted A1 - Hazra, Shuvendu A1 - Thakur, R P A1 - Devi, G U A1 - Mathur, K TI - Pathogenic and Molecular Variability Among Twelve Isolates of Colletotrichum graminicola from Sorghum UR - http://oar.icrisat.org/4937/ JF - Indian Journal of Mycology & Plant Pathology SN - 0303-4097 PB - Indian Society of Mycology and Plant Pathology N1 - The senior author is highly thankful to Dr. S. Sivaramakrishnan, Scientist (Biochemistry), ICRISAT for his guidance to conduct molecular work and to Mr. K.D.V. Prasad for his help to prepare dendrograms. N2 - Variability in 12 foliar and grain isolates of sorghum anthracnose pathogen Colletotrichum graminicola was studied through virulence and Random Amplified Polymorphic DNA ( RAPD) assay. For virulence analysis, pot grown 21-day-old plants of six differential sorghum lines were spray inoculated with conidia (1 x 105 conidia ml-1). The isolates showed significant (P< 0.05) variations in latent period, aggressiveness and virulence. Overall pathogenic potential was determined as Virulence Index. Hierarchical clustering using euclidean test based on "Virulence Index" grouped the 12 isolates into three groups, while that based on RAPDs using primer OPA8 grouped these into four groups. Monoconidial isolate Cg 226-3 had 85% genetic and pathogenic similarity like its parent, while Cg 227-3 showed virulence variation and 65% genetic similarity with its parent, indicating heterogeneity in Cg 227. KW - Sorghum bicolor KW - Colletotrichum graminicola KW - virulence KW - pathotypes KW - DNA polymorphism Y1 - 1999/// SP - 176 ID - icrisat4937 EP - 183 VL - 29 IS - 2 ER -