eprintid: 4777
rev_number: 10
eprint_status: archive
userid: 30
dir: disk0/00/00/47/77
datestamp: 2011-12-03 12:25:58
lastmod: 2011-12-03 12:25:58
status_changed: 2011-12-03 12:25:58
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Sharma, K K
creators_name: Bhojwani, S S
icrisatcreators_name: Sharma, K K
affiliation: University of Delhi(Delhi)
affiliation: ICRISAT(Patancheru)
country: India
title: Histological aspects of in vitro root and shoot differentiation from cotyledon explants of Brassica juncea (L.) czern
ispublished: pub
subjects: s2.16
subjects: s2.4
full_text_status: restricted
keywords: Brassica juncea; cotyledon culture; histology; organogenesis; regeneration; tissue culture


abstract: Under fairly simple culture conditions, excised cotyledons of Brassica juncea undergo high frequencies of either root formation on growth regulator-free Murashige and Skoog (MS) medium or shoot formation on MS + 5.0 μM N6-benzyladenine (BA). Temporal histological events of organ differentiation processes revealed that while root formation was endogenous in origin and did not involve callusing, shoot bud differentiation occurred after the formation of meristematic nodules at the cut end. While root primordia differentiated from the petiolar cut end after 4 days in culture, the formation of a meristematic nodule at the same end occurred by 4–6 days which further lead to the differentiation of shoot meristem by 8–10 days. Although the initial accumulation of starch in organogenic cotyledons was the same during both root and shoot formation, its depletion was faster in shoot forming cultures suggesting that this process is more energy requiring than rhizogenesis.


date: 1990
date_type: published
publication: Plant Science
volume: 69
number: 2
publisher: Elsevier
pagerange: 207-214
refereed: TRUE
issn: 0168-9452
official_url: http://dx.doi.org/10.1016/0168-9452(90)90119-9 
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22Histological+aspects+of+in+vitro+root+and+shoot+differentiation+from+cotyledon+explants+of+Brassica+juncea+%28L.%29+czern%22&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
citation:   Sharma, K K and Bhojwani, S S  (1990) Histological aspects of in vitro root and shoot differentiation from cotyledon explants of Brassica juncea (L.) czern.  Plant Science, 69 (2).  pp. 207-214.  ISSN 0168-9452     
document_url: http://oar.icrisat.org/4777/1/Plant_Sc_69_2_1990_207-214.pdf