<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Isolation and Characterization of Baculoviruses from Three\r\nMajor Lepidopteran Pests in the Semi-Arid Tropics of India"^^ . "Baculoviruses were isolated from three major\r\nlepidopteran pests, Helicoverpa armigera, Spodoptera litura\r\nand Amsacta albistriga in the semi-arid tropics during\r\nnatural epizootic conditions at ICRISAT fields, Patancheru,\r\nAndhra Pradesh, India. Biological, morphological and\r\nbiochemical analysis identified these isolates as Nucleopolyhedroviruses\r\n(NPVs). Scanning electron microscopy of\r\nthe occlusion bodies (OBs) purified from diseased larvae\r\nrevealed polyhedral particles of size approximately\r\n0.5–2.5 lm [Helicoverpa armigera Nucleopolyhedrovirus\r\n(HearNPV)], 0.9–2.92 lm [Spodoptera litura Nucleopolyhedrovirus\r\n(SpltNPV)] and 1.0–2.0 lm [Amsacta albistriga\r\nNucleopolyhedrovirus (AmalNPV)] in diameter. Transmission\r\nelectron microscopy of thin sections of OBs of the three\r\nisolates revealed up to 5–8 multiple bacilliform shaped\r\nparticles packaged within a single viral envelope. The\r\ndimensions of these particles were 277.7 9 41.6 nm for\r\nHearNPV, 285.7 9 34.2 nm for SpltNPV and 228.5 9\r\n22.8 nm for AmalNPV. Each of HearNPV and AmalNPV\r\ncontained up to 6 nucleocapsids and SpltNPV contained up\r\nto 7 nucleocapsids per envelope. The estimated molecular\r\nweights of the purified OB (polyhedrin) protein of the three\r\nNPVs were 31.29–31.67 kDa. Virus yield (OBs/larva) was\r\n5.18 ± 0.45 9 109 for HearNPV, 5.73 ± 0.17 9 109 for\r\nSpltNPV and 7.90 ± 0.54 9 109 for AmalNPV. The LC50\r\nvalues of various NPVs against 2nd and 3rd instar larvae\r\nindicated 2.30 9 104 and 1.5 9 105 OBs/ml for HearNPV,\r\n3.5 9 104 and 2.4 9 105 OBs/ml for SpltNPV and 5.6 9\r\n104 and 3.96 9 105 OBs/ml for AmalNPV. The lethal time\r\nrequired to cause 50% mortality (LT50) for these three species\r\nwere also defined. This study has shown that the NPVs\r\ninfecting three major lepidopteran pests in India are multiple\r\nNPVs, and they have good potential to use as biocontrol\r\nagents against these important pests."^^ . "2011" . . "22" . "1" . . "Indian Virological Society"^^ . . . "Indian Journal of Virology"^^ . . . "09702822" . . . . . . . . . . . . . . . . "G V"^^ . "Ranga Rao"^^ . "G V Ranga Rao"^^ . . "K"^^ . "Sireesha"^^ . "K Sireesha"^^ . . "P L"^^ . "Kumar"^^ . "P L Kumar"^^ . . "Ch"^^ . "Sridhar Kumar"^^ . "Ch Sridhar Kumar"^^ . . . . . . "Isolation and Characterization of Baculoviruses from Three\r\nMajor Lepidopteran Pests in the Semi-Arid Tropics of India (PDF)"^^ . . . . . . . . "Isolation and Characterization of Baculoviruses from Three\r\nMajor Lepidopteran Pests in the Semi-Arid Tropics of India (Indexer Terms)"^^ . . . . . . "Isolation and Characterization of Baculoviruses from Three\r\nMajor Lepidopteran Pests in the Semi-Arid Tropics of India (Image (JPEG))"^^ . . . . . "HTML Summary of #44 \n\nIsolation and Characterization of Baculoviruses from Three \nMajor Lepidopteran Pests in the Semi-Arid Tropics of India\n\n" . "text/html" . . . "Entomology"@en . .