eprintid: 3969
rev_number: 8
eprint_status: archive
userid: 19
dir: disk0/00/00/39/69
datestamp: 2011-11-17 03:34:40
lastmod: 2011-11-17 03:34:40
status_changed: 2011-11-17 03:34:40
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Kishore, G K
creators_name: Pande, S
creators_name: Podile, A R
icrisatcreators_name: Kishore, G K
icrisatcreators_name: Pande, S
affiliation: ICRISAT(Patancheru)
affiliation: University of Hyderabad
country: India
title: Biological control of late leaf spot of peanut (Arachis hypogaea) with chitinolytic bacteria
ispublished: pub
subjects: s1.3
full_text_status: public
keywords: Biotrophic, phylloplane, rhizobacteria
note: The Andhra Pradesh-Netherlands Biotechnology Programme supported this work in the form of a research grant to A. R. Podile and senior research fellowship to G. K. Kishore. We thank the UGC-SAP and DSTFIST programmes of the Department of Plant Sciences, University of Hyderabad
abstract: Late leaf spot (LLS), caused by Phaeoisariopsis personata, is a foliar disease of groundnut or peanut (Arachis hypogaea) with high economic and global importance. Antifungal and chitinolytic Bacillus circulans GRS 243 and Serratia marcescens GPS 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of LLS. Chitin-supplemented application of B. circulans GRS 243 and S. marcescens GPS 5 resulted in improved biological control of LLS disease. Supplementation of bacterial cells with 1% (wt/vol) colloidal chitin reduced lesion frequency by 60% compared with application of bacterial cells alone, in the greenhouse. Chitin-supplemented application of GRS 243 and GPS 5 also resulted in improved and stable control of LLS in a repeated field experiment and increased the pod yields by 62 and 75%, respectively, compared with the control. Chitin-supplemented application of GPS 5 was tested in six on-farm trials, and the increase in pod yields was up to 48% in kharif (rainy season). A 55-kDa chitinase was purified from the cell-free culture filtrate of GPS 5 by affinity chromatography and gel filtration. Purified chitinase of S. marcescens GPS 5 (specific activity 120 units) inhibited the in vitro germination of P. personata conidia, lysed the conidia, and effectively controlled LLS in greenhouse tests, indicating the importance of chitinolysis in biological control of LLS disease by GPS 5
date: 2005
date_type: published
publication: Phytopathology 
volume: 95
number: 10
publisher: American Phytopathological Society
pagerange: 1157-1165
refereed: TRUE
issn: 0031-949X
official_url: http://dx.doi.org/10.1094/PHYTO-95-1157
related_url_url: http://scholar.google.co.in/scholar?as_q=Biological+control+of+late+leaf+spot+of+peanut+%28Arachis+hypogaea%29+with+chitinolytic+bacteria&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.
related_url_type: author
funders: Andhra Pradesh-Netherlands Biotechnology Programme
citation:   Kishore, G K and Pande, S and Podile, A R  (2005) Biological control of late leaf spot of peanut (Arachis hypogaea) with chitinolytic bacteria.  Phytopathology , 95 (10).  pp. 1157-1165.  ISSN 0031-949X     
document_url: http://oar.icrisat.org/3969/1/Phytopathology_95_10_1157-1165_2005.pdf