eprintid: 37
rev_number: 14
eprint_status: archive
userid: 1
dir: disk0/00/00/00/37
datestamp: 2011-05-25 11:07:44
lastmod: 2012-06-11 03:07:28
status_changed: 2011-05-25 11:07:44
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Yadav, R S
creators_name: Sehgal, D
creators_name: Vadez, V
icrisatcreators_name: Vadez, V
affiliation: Aberystwyth University(Gogerddan)
affiliation: ICRISAT(Patancheru)
country: UK
country: India
title: Using genetic mapping and genomics approaches in
understanding and improving drought tolerance in pearl
millet
ispublished: pub
subjects: S1.5
full_text_status: restricted
keywords: Fine mapping, gene-based markers, genetic mapping, germplasm population, high-resolution cross,
marker-assisted backcrossing; pearl millet; QTL validation; QTLs; terminal drought tolerance
agrotags: Agrotags - yields | drought | grain | millets | pearl millet (plant) | tolerance | environment | genetics | social groups | planting
Fishtags - pearls | drying
Geopoliticaltags - usa | niger | africa | india
note: The authors wish to acknowledge the Biotechnology and
Biological Sciences Research Council (BBSRC) and the
Department for International Development (DFID) for
funding to their work via grant number BB/F004133/1.
They also thank the anonymous reviewers for their helpful
comments and suggestions.
abstract: Drought at the reproductive stage is a major constraint to pearl millet [Pennisetum glaucum (L.) R. Br.] productivity.
Quantitative trait locus (QTL) mapping provides a means to dissect complex traits, such as drought tolerance, into
their components, each of which is controlled by QTLs. Molecular marker-supported genotypic information at the
identified QTLs then enables quick and accurate accumulation of desirable alleles in plant breeding programmes.
Recent genetic mapping research in pearl millet has mapped several QTLs for grain yield and its components under
terminal drought stress conditions. Most importantly, a major QTL associated with grain yield and for the drought
tolerance of grain yield in drought stress environments has been identified on linkage group 2 (LG 2) which accounts
for up to 32% of the phenotypic variation of grain yield in mapping population testcrosses. The effect of this QTL has
been validated in two independent marker-assisted backcrossing programmes, where 30% improvement in grain
yield general combining ability (GCA) expected of this QTL under terminal drought stress conditions was recovered
in the QTL introgression lines. To transfer effectively favourable alleles of this QTL into pearl millet varieties that
otherwise are high yielding and adapted to specific agricultural zones, efforts are currently underway to develop
closely spaced gene-based markers within this drought tolerance (DT)-QTL. In this review, an overview is provided
of information on the genetic maps developed in pearl millet for mapping drought tolerance traits and their
applications in identifying and characterizing DT-QTLs. Marker-assisted transfer of desirable QTL alleles to elite
parent backgrounds, and results from introgression line validation in multiple terminal drought stress environments
are discussed. Current efforts undertaken towards delimiting the interval of a major DT-QTL mapping to LG 2, and
towards identifying candidate genes and physiologies underlying this QTL are presented. Highly specialized genetic
stocks [QTL-near-isogenic lines (NILs), a high-resolution cross, and a germplasm population] and genomic
resources (gene sequences, gene-based markers, and comparative genomics information) specifically developed
for these purposes are discussed.
date: 2011
publication: Journal of Experimental Botany
volume: 62
number: 2
publisher: Oxford University Press
pagerange: 397-408
refereed: TRUE
official_url: http://dx.doi.org/10.1093/jxb/erq265
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+Using+genetic+mapping+and+genomics+approaches+in+understanding+and+improving+drought+tolerance+in+pearl+millet&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
citation: Yadav, R S and Sehgal, D and Vadez, V (2011) Using genetic mapping and genomics approaches in understanding and improving drought tolerance in pearl millet. Journal of Experimental Botany, 62 (2). pp. 397-408.
document_url: http://oar.icrisat.org/37/1/Yadav-Sehgal-Vadez_2011_-_JXB.pdf