%0 Journal Article %@ 0974-0120 %A Manohar, S K %A Rajeshwari, R %D 1985 %F icrisat:3510 %I Indian Virological Society %J Indian Journal of Virology %N 2 %P 157-162 %T Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses %U http://oar.icrisat.org/3510/ %V 1 %X Four negative stains were tested on Peanut mottle virus (PMV), two irolater of Peanut green mosaic virus-systemic necrosis (PGMV-SN) ar~dsy etrmic mosaic (PGM V-SM), to datrrmlne their effects on the stability and particle length distribution. Whan phosphotunngstate(PleTA ) at pH 7.0 was used, particle lengh and width of PMV and PGMV-SM werr conside~ably affecrnd rasulting in variation, whereas PGMV-SN war la18 affected. When uranyl acotete (UA) and urrnyl formate (UF) ware ured, tho prrlicler of PGMV-SM and PMV were better preserved, in contrast to PGMV-SN which was unstable in both the uranyl stains. PGMV.SN was well presarved in ammonium molybdrte (AM) o f pHs 3.5 and 6.5 but was d;sruoted in OH 8.0. Although PMV could be stairled with AM of pH 3.6, particles ware poorly stained In wM ot pHs 6.6 and 8.0. Pertlcles ol PGMV-SM were poorly strined in AM of all the three pH valuer.