eprintid: 3371
rev_number: 16
eprint_status: archive
userid: 65
dir: disk0/00/00/33/71
datestamp: 2011-11-13 11:49:02
lastmod: 2012-11-30 07:53:29
status_changed: 2011-11-13 11:49:02
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Rajeshwari, R
creators_name: Iizuka, N
creators_name: Nolt, B L
icrisatcreators_name: Rajeshwari, R
icrisatcreators_name: Iizuka, N
icrisatcreators_name: Nolt, B L
affiliation: ICRISAT(Patancheru)
country: India
title: Purification, serology and physico-chemical properties of a peanut mottle virus isolate from India
ispublished: pub
subjects: s1.2
full_text_status: restricted
abstract: A procedure developed for the purification of peanut mottle virus (PMV) isolated from peanuts in India yielded 30–40 mg of virus/kg of plant tissue. Purified virus sedimented as a single component of 151 S. The virus coat protein migrated as a single component in each of two polyacrylamide gel concentrations and had an estimated molecular weight of 34 000 daltons. The molecular weight of the nucleic acid was 3.10 × 106 daltons. In the precipitin ring interface test (PRIT) PMV showed a serological relationship with soybean mosaic virus (SMV). Using the double antibody sandwich form of ELISA, PMV was shown to be distantly related to adzuki bean mosaic (ABMV), amaranthus leaf mottle (ALMV), clover yellow vein (CYVV) viruses and SMV. Immunosorbent electron microscopy (ISEM) showed PMV to be closely related to ABMV, ALMV, CYVV and SMV. In PRIT, ELISA and ISEM tests no specific serological reaction was noted between PMV and antisera to groundnut eye spot, peanut green mosaic, pepper veinal mottle, potato virus Y, sugarcane mosaic and turnip mosaic viruses.
date: 1983
date_type: published
publication: Plant Pathology
volume: 1983
number: 132
publisher: Blackwell Publishing
pagerange: 197-205
refereed: TRUE
issn: 1365-3059
official_url: http://dx.doi.org/10.1111/j.1365-3059.1983.tb01319.x
related_url_url: http://scholar.google.co.in/scholar?as_q=%22Purification%2C+serology+and+physico-chemical+properties+of+a+peanut+mottle+virus+isolate+from+India%22&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Rajeshwari, R and Iizuka, N and Nolt, B L  (1983) Purification, serology and physico-chemical properties of a peanut mottle virus isolate from India.  Plant Pathology, 1983 (132).  pp. 197-205.  ISSN 1365-3059     
document_url: http://oar.icrisat.org/3371/1/Plant_path_32_197-205_1983.pdf