eprintid: 3221
rev_number: 7
eprint_status: archive
userid: 19
dir: disk0/00/00/32/21
datestamp: 2011-10-28 04:17:40
lastmod: 2011-10-28 04:17:40
status_changed: 2011-10-28 04:17:40
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Kishore, G K
creators_name: Pande, S
creators_name: Podile, A R
icrisatcreators_name: Pande, S
affiliation: ICRISAT(Patancheru)
affiliation: University of Hyderabad
country: India
title: Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa
ispublished: pub
subjects: s1.3
full_text_status: restricted
keywords: Arachis hypogaea, integrated disease management, peanut,
Phaeoisariopsis personata
note: This work was supported in the form of a research grant
by Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Pubic Enterprises, Hyderabad, India to ARP, and Department for International Development (DFID), UK, to SP. GKK thanks the APNLBP for the award of a Senior Research Fellowship. ARP and GKK are grateful to the UGC-SAP and DST-FIST programs of the Department of Plant Sciences, University of Hyderabad, Hyderabad, for providing the research facilities
abstract: Fifteen groundnut-associated bacterial isolates that inhibited by >90% the in vitro conidial germination of Phaeoisariopsis personata, causal agent of late leaf spot disease of groundnut, were applied as a prophylactic spray (108 cfu mL-1) and tested for control of the disease in the glasshouse. Two groundnut seed-associated bacterial isolates, GSE 18 and GSE 19, identified as Pseudomonas aeruginosa, reduced the lesion frequency (LF) by up to 70%. A 90-day-old peat-based formulation of P. aeruginosa GSE 18 reduced LF measured 15 days postinoculation by up to 60%. Both P. aeruginosa GSE 18 and GSE 19 were tolerant to chlorothalonil (Kavach®) up to 2000 µg mL-1 in LB broth. In glasshouse trials, GSE 18 and GSE 19 tested in combination with reduced concentrations of chlorothalonil were highly efficient in management of the disease. The disease was completely controlled by chlorothalonil (>250 µg mL-1), and in the presence of GSE 18 or GSE 19, 100 µg mL-1 chlorothalonil was equally effective. Application of rifamycin-resistant mutants of GSE 18 or GSE 19 together with chlorothalonil significantly increased the survival of these isolates in the groundnut phylloplane. In the field, a combination of GSE 18 and 500 µg mL-1 chlorothalonil reduced disease severity comparable to 2000 µg mL-1 chlorothalonil alone. Use of chlorothalonil-tolerant pseudomonads together with a quarter concentration of the recommended field dose of chlorothalonil doubled pod yield compared with the untreated unsprayed control
date: 2005
date_type: published
publication: Plant Pathology
volume: 54
number: 3
publisher: Blackwell Publishing
pagerange: 401-408
refereed: TRUE
issn: 0032-0862
official_url: http://dx.doi.org/10.1111/j.1365-3059.2005.01160.x
related_url_url: http://scholar.google.co.in/scholar?q=IManagement+of+late+leaf+spot+of+groundnut+%28Arachis+hypogaea%29+with+chlorothalonil-tolerant+isolates+of+Pseudomonas+aeruginosa&hl=en&btnG=Search&as_sdt=1%2C5&as_sdtp=on
related_url_type: author
funders: Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Public Enterprises, Hyderabad India to ARP, and Department for International Development (DFID), UK, to SP
citation:   Kishore, G K and Pande, S and Podile, A R  (2005) Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa.  Plant Pathology, 54 (3).  pp. 401-408.  ISSN 0032-0862     
document_url: http://oar.icrisat.org/3221/1/PlantPathology_54_3_401-408_2005.pdf