"3221","7","archive","19",,,"disk0/00/00/32/21","2011-10-28 04:17:40","2011-10-28 04:17:40","2011-10-28 04:17:40","article",,,"show","Library-ICRISAT@CGIAR.ORG",,,"","","","","","","","","","","0",,,"Kishore","G K","","","","","","","Pande","S","","","","",,,,,"","",,,,,"","","ICRISAT(Patancheru)","India","Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa","pub","s1.3","","","restricted",,,"Arachis hypogaea, integrated disease management, peanut,
Phaeoisariopsis personata",,"This work was supported in the form of a research grant
by Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Pubic Enterprises, Hyderabad, India to ARP, and Department for International Development (DFID), UK, to SP. GKK thanks the APNLBP for the award of a Senior Research Fellowship. ARP and GKK are grateful to the UGC-SAP and DST-FIST programs of the Department of Plant Sciences, University of Hyderabad, Hyderabad, for providing the research facilities","Fifteen groundnut-associated bacterial isolates that inhibited by >90% the in vitro conidial germination of Phaeoisariopsis personata, causal agent of late leaf spot disease of groundnut, were applied as a prophylactic spray (108 cfu mL-1) and tested for control of the disease in the glasshouse. Two groundnut seed-associated bacterial isolates, GSE 18 and GSE 19, identified as Pseudomonas aeruginosa, reduced the lesion frequency (LF) by up to 70%. A 90-day-old peat-based formulation of P. aeruginosa GSE 18 reduced LF measured 15 days postinoculation by up to 60%. Both P. aeruginosa GSE 18 and GSE 19 were tolerant to chlorothalonil (Kavach®) up to 2000 µg mL-1 in LB broth. In glasshouse trials, GSE 18 and GSE 19 tested in combination with reduced concentrations of chlorothalonil were highly efficient in management of the disease. The disease was completely controlled by chlorothalonil (>250 µg mL-1), and in the presence of GSE 18 or GSE 19, 100 µg mL-1 chlorothalonil was equally effective. Application of rifamycin-resistant mutants of GSE 18 or GSE 19 together with chlorothalonil significantly increased the survival of these isolates in the groundnut phylloplane. In the field, a combination of GSE 18 and 500 µg mL-1 chlorothalonil reduced disease severity comparable to 2000 µg mL-1 chlorothalonil alone. Use of chlorothalonil-tolerant pseudomonads together with a quarter concentration of the recommended field dose of chlorothalonil doubled pod yield compared with the untreated unsprayed control","2005","published",,"Plant Pathology","54","3","Blackwell Publishing",,"401-408",,,,,,,,,,,"TRUE",,"0032-0862",,,,,,"","http://dx.doi.org/10.1111/j.1365-3059.2005.01160.x","http://scholar.google.co.in/scholar?q=IManagement+of+late+leaf+spot+of+groundnut+%28Arachis+hypogaea%29+with+chlorothalonil-tolerant+isolates+of+Pseudomonas+aeruginosa&hl=en&btnG=Search&as_sdt=1%2C5&as_sdtp=on","author",,"Andhra Pradesh-Netherlands Biotechnology Program (APNLBP), Institute of Public Enterprises, Hyderabad India to ARP, and Department for International Development (DFID), UK, to SP","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"3221",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Pande","S","","",,,,,,,,,,,,,,,,,,,,,,,"University of Hyderabad",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"3221",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Podile","A R","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,