eprintid: 3187
rev_number: 11
eprint_status: archive
userid: 19
dir: disk0/00/00/31/87
datestamp: 2011-10-25 11:02:38
lastmod: 2011-12-06 10:57:38
status_changed: 2011-10-25 11:02:38
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Jayashree, B
creators_name: Buhariwalla, H K
creators_name: Shinde, S
creators_name: Crouch, J H
icrisatcreators_name: Jayashree, B
icrisatcreators_name: Buhariwalla, H K
icrisatcreators_name: Shinde, S
icrisatcreators_name: Crouch, J H
affiliation: ICRISAT(Patancheru)
country: India
title: A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database
ispublished: pub
subjects: s1.1
full_text_status: restricted
keywords: cloning, data mining, drought avoidance, drought tolerance, EST database, root traits, stress
note: The authors gratefully acknowledge the programming
support extended by Mr. P. Vinod Kumar and Ms. R.
Rajalakshmi
abstract: Chickpea, a lesser-studied grain legume, is being investigated due to its taxonomic proximity with the model legume genome Medicago truncatula and its ability to endure and grow in relatively low soil water contents making it a model legume crop for the study of agronomic response to drought stress. Public databases currently contain very few sequences from chickpea associated with expression in root tissues. However, root traits are likely to be one of the most important components of drought tolerance in chickpea. Thus, we have generated a set of over 2800 chickpea expressed sequence tags (ESTs) from a library constructed after subtractive suppressive hybridization (SSH) of root tissue from two closely related chickpea genotypes possessing different sources of drought avoidance and tolerance (ICC4958 and Annigeri respectively). This database provides researchers in legume genomics with a major new resource for data mining associated with root traits and drought tolerance. This report describes the development and utilization of the database and provides the tools we have developed to facilitate the bioinformatics pipeline used for analysis of the ESTs in this database. We also discuss applications that have already been achieved using this resource
date: 2005
date_type: published
publication: Electronic Journal of Biotechnology
volume: 8
number: 2
publisher: Universidad Católica de Valparaíso
pagerange: 128-133
refereed: TRUE
issn: 0717-3458
official_url: http://dx.doi.org/10.2225/vol8-issue2-fulltext-8
related_url_url: http://scholar.google.co.in/scholar?as_q=%22A+legume+genomics+resource%3A+The+Chickpea+Root+Expressed+Sequence+Tag+Database%22&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_sdtp=on
related_url_type: author
funders: Governments of UK, Japan, European Union and the Generation Challenge Program
citation:   Jayashree, B and Buhariwalla, H K and Shinde, S and Crouch, J H  (2005) A legume genomics resource: The Chickpea Root Expressed Sequence Tag Database.  Electronic Journal of Biotechnology, 8 (2).  pp. 128-133.  ISSN 0717-3458     
document_url: http://oar.icrisat.org/3187/1/EJB_8_2_128-133_2005.pdf