@article{icrisat3142, year = {1994}, title = {Seedborne infection of Eleusine coracana by Bipolaris nodulosa and Pyricularia grisea in Uganda and Kenya}, volume = {78}, number = {1}, pages = {60--63}, author = {S Pande and S Z Mukuru and R O Odhiambo and R I Karunakar}, publisher = {American Phytopathological Society}, journal = {Plant Disease}, url = {http://oar.icrisat.org/3142/}, abstract = {Seed samples of finger millet (Eleusine coracana) collected from Uganda and Kenya had mixed infections with Bipolaris nodulosa (4-49\%) and Pyricularia grisea (7-55\%). Most samples showed poor germination and were unfit for sowing because of a high percentage of Seedborne infection. We obtained similar seed infection counts with the standard blotter, deep-freezing blotter, and agar plate methods, which suggests that any of these methods could be used for routine seed health testing. However, sporulation was better on frozen seeds. Gray or black discolored seeds had higher levels of infection by P. grisea than apparently healthy, normal reddish brown seeds. Plating of seed components showed that both fungi were present in the pericarp and endosperm but not in the embryo. Seed transmission tests demonstrated the ability of these fungi to kill young seedlings. }, keywords = {Seed samples of finger millet (Eleusine coracana) collected from Uganda and Kenya had mixed infections with Bipolaris nodulosa (4-49\%) and Pyricularia grisea (7-55\%). Most samples showed poor germination and were unfit for sowing because of a high percentage of Seedborne infection. We obtained similar seed infection counts with the standard blotter, deep-freezing blotter, and agar plate methods, which suggests that any of these methods could be used for routine seed health testing. However, sporulation was better on frozen seeds. Gray or black discolored seeds had higher levels of infection by P. grisea than apparently healthy, normal reddish brown seeds. Plating of seed components showed that both fungi were present in the pericarp and endosperm but not in the embryo. Seed transmission tests demonstrated the ability of these fungi to kill young seedlings. } }