OAR@ICRISATDevelopment of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpeaSPandeauthorMSharmaauthorP MGaurauthorSTripathiauthorLKaurauthorABasandraiauthorTKhanauthorC L LGowdaauthorK H MSiddiqueauthorEffective controlled-environment and field screening techniques were developed and refined to identify resistance to Ascochyta blight (AB), caused by Ascochyta rabiei (Pass.) Labr. in chickpea. A controlled environment plant growth room facility developed for AB evaluation at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India was modified to evaluate chickpea genotypes for resistance to AB. Controlled environment screening techniques, such as a seedling screening technique using 10-day-old seedlings and cut-twig screening techniques using excised twigs (10–15cm long) were developed. Components of the screening techniques were optimized in the controlled environment-plant growth room. The controlled environment screening techniques were found to be rapid, reliable and reproducible and a positive correlation was found between the seedling and cut-twig screening techniques (r=0.94). The cut-twig screening technique was quicker than the seedling screening technique and is particularly useful in screening segregating breeding lines derived from wild Cicer spp. Results of the controlled environment screening techniques were compared with results of field screening trials carried out at Dhaulakuan and Ludhiana in India, where the pathogen is endemic. A significant positive correlation was found between results from the controlled environment and field screening techniques (r=0.88). Using these resistance screening techniques, 150 elite chickpea breeding lines were evaluated and 29 lines with high and stable resistance to AB were identified.Chickpea2011CSIRO PublishingArticle

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.