eprintid: 2984
rev_number: 12
eprint_status: archive
userid: 30
dir: disk0/00/00/29/84
datestamp: 2011-10-20 07:53:14
lastmod: 2011-10-20 07:53:14
status_changed: 2011-10-20 07:53:14
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Devi, K T
creators_name: Mayo, M A
creators_name: Hall, A J
creators_name: Craufurd, P Q
creators_name: Wheeler, T R
creators_name: Waliyar, F
creators_name: Subramanyam, A
creators_name: Reddy, D V R
icrisatcreators_name: Devi, K T
icrisatcreators_name: Hall, A J
icrisatcreators_name: Waliyar, F
icrisatcreators_name: Subramanyam, A
icrisatcreators_name: Reddy, D V R
affiliation: ICRISAT(Patancheru)
affiliation: Scottish Crop Research Institute(Dundee)
affiliation: University of Reading(Reading)
country: India
country: UK
title: Development and Application of an Indirect Competitive
Enzyme-Linked Immunoassay for Aflatoxin M1 in Milk and   Milk-Based Confectionery

ispublished: pub
subjects: s1.3
subjects: s2.17
full_text_status: restricted
keywords: Aflatoxin M1, antibodies, ELISA, milk, milk-based confectionery
note: We thank Y. Ramachandraiah for technical assistance.
abstract: High-titer rabbit polyclonal antibodies to aflatoxin M1 (AFM1) were produced by utilizing AFM1-
bovine serum albumin (BSA) conjugate as an immunogen. An indirect competitive enzyme-linked
immunosorbent assay was standardized for estimating AFM1 in milk and milk products. To avoid the
influence of interfering substances present in the milk samples, it was necessary to prepare AFM1
standards in methanol extracts of certified reference material (CRM) not containing detectable AFM1
(<0.05 ng/g). The reliability of the procedure was assessed by using CRM with AFM1 concentrations
of <0.5 and 0.76 ng/g. Also, assays of milk samples mixed with AFM1 ranging in concentration
between 0.5 and 50 ng/L gave recoveries of >93%. The relative cross-reactivity with aflatoxins (AF)
and ochratoxin A, assessed as the amount of AFM1 necessary to cause 50% inhibition of binding,
was 5% for AFB1 and much less for AFB2, AFG1, and AFG2; there was no reaction with ochratoxin
A. AFM1 contamination was measured in retail milk and milk products collected from rural and
periurban areas in Andhra Pradesh, India. Of 280 milk samples tested, 146 were found to contain
<0.5 ng/mL of AFM1; in 80 samples it varied from 0.6 to 15 ng/mL, in 42 samples from 16 to 30
ng/mL, and in 12 samples from 31 to 48 ng/mL. Most of the milk samples that contained high AFM1
concentrations were obtained from periurban locations. The results revealed a significant exposure
of humans to AFM1 levels in India and thus highlight the need for awareness of risk among milk
producers and consumers.

date: 2000
date_type: published
publication: Journal of Agriculture and Food Chemistry
volume: 50
publisher: American Chemical Society
pagerange: 933-937
refereed: TRUE
issn: 0021-8561
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22+Development+and+Application+of+an+Indirect+Competitive+Enzyme-Linked+Immunoassay+for+Aflatoxin+M1+in+Milk+and++++++++++++++++Milk-Based+Confectionery%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=
related_url_type: author
citation:   Devi, K T and Mayo, M A and Hall, A J and Craufurd, P Q and Wheeler, T R and Waliyar, F and Subramanyam, A and Reddy, D V R  (2000) Development and Application of an Indirect Competitive Enzyme-Linked Immunoassay for Aflatoxin M1 in Milk and Milk-Based Confectionery.  Journal of Agriculture and Food Chemistry, 50.  pp. 933-937.  ISSN 0021-8561     
document_url: http://oar.icrisat.org/2984/1/Development_and_application_of_an_indirect_competitive_enzyme-linked_immunoassay_for_aflatoxin_M-1_in_milk_and_milk-based.pdf