"2972","12","archive","30",,,"disk0/00/00/29/72","2011-10-19 13:52:33","2011-10-19 13:52:33","2011-10-19 13:52:33","article",,,"show","Library-ICRISAT@CGIAR.ORG",,,"","","","","","","","","","","0",,,"Buhariwalla","H K","","","","","","","Srilakshmi","P","","","","",,,,,"","",,,,,"","","Centro Internacional de Mejoramiento de Maiz y Trigo(Apdo)","Mexico","AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics","pub","s1.3","","","restricted",,,"Trichoderma, amplified fragment length polymorphism, internal transcribed spacer, aflatoxin, groundnut",,"This work was supported by ICRISAT core funds including ear-
marked unrestricted grants from the governments of the UK, Japan
and The European Union. The manuscript was greatly improved by
comments from Dr John Leslie (Kansas State University). We thank
B. Moss and K. Eshwar for assistance with DNA extractions, AFLP
analysis and sequencing; Ramdas, Malareddy and Bukama for gen-
eral laboratory assistance; P.V.N.S. Prasad and K.D.V. Prasad for
assistance with figures and Dr Rolf Folkerstma (ICRISAT, Kenya)
for reviewing the manuscript. The AFLP and antagonistic data used
for comparative analysis in this report form part of the Doctoral
thesis of Ms P. Srilakshmi.
","Trichoderma species offer considerable potential for controlling aflatoxin contamination in groundnut and other crops. Initial classification of 48 Trichoderma isolates, derived from four different groundnut cultivation sites in India was based on alignment of 28S rDNA sequences to GenBank sequences of ex-type strains. This was found to be substantially more reliable than our routine morphological characterization, but did not provide a comprehensive diagnostic solution, as unique single nucleotide polymorphism (SNP) haplotypes could not be identified for all species. However, all the Trichoderma isolates could be readily distinguished by amplified fragment length polymorphism (AFLP) analysis, based on six primer pair combinations, which generated 234 polymorphic bands. In addition, individual AFLP bands were identified which differentiate closely related species. Similarly, AFLP bands were identified that correlated with different types of antagonism to Aspergillus flavus. The implications of these results for the development of simple polymerase chain reaction (PCR)-based diagnostic assays for antagonistic isolates of Trichoderma is discussed.","2005","published",,"Journal Of Phytopathology","153","7-8","Blackwell Publishing",,"389-400",,,,,,,,,,,"TRUE",,"1439-0434",,,,,,"","http://dx.doi.org/10.1111/j.1439-0434.2005.00989.x","http://scholar.google.co.in/scholar?as_q=%22AFLP+Analysis+of+Trichoderma+spp.+from+India+Compared+with+Sequence+and+Morphological-based+Diagnostics%22&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_y","author",,"Government of UK","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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