@article{icrisat2811, publisher = {International Crops Research Institute for the Semi-Arid Tropics}, year = {1994}, title = {A Technique for Phytophthora drechsleri f. sp. cajani Sporangia and Zoospores Production}, pages = {36--37}, author = {J Kannaiyan and V K Sheila and Y L Nene}, volume = {1}, journal = {International Chickpea Newsletter}, url = {http://oar.icrisat.org/2811/}, abstract = {Cultures on V-8 juice agar medium were incubated for 5 d at 30?C, then 5 mm diam. discs were removed from the periphery of the colonies and floated in clear V-8 juice and sterile distilled water (1:5) for a further 24 h at 30? under fluorescent light. The mycelial discs were rinsed twice in sterile distilled water and incubated again under the same conditions in sterile distilled water. Abundant sporangia were produced in 16 h and suspensions of c. 50 000 zoospores/ml were obtained.} }