<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)"^^ . "Background: Cultivated peanut or groundnut (Arachis hypogaea L.) is the fourth most important oilseed\r\ncrop in the world, grown mainly in tropical, subtropical and warm temperate climates. Due to its origin\r\nthrough a single and recent polyploidization event, followed by successive selection during breeding\r\nefforts, cultivated groundnut has a limited genetic background. In such species, microsatellite or simple\r\nsequence repeat (SSR) markers are very informative and useful for breeding applications. The low level of\r\npolymorphism in cultivated germplasm, however, warrants a need of larger number of polymorphic\r\nmicrosatellite markers for cultivated groundnut.\r\nResults: A microsatellite-enriched library was constructed from the genotype TMV2. Sequencing of 720\r\nputative SSR-positive clones from a total of 3,072 provided 490 SSRs. 71.2% of these SSRs were perfect\r\ntype, 13.1% were imperfect and 15.7% were compound. Among these SSRs, the GT/CA repeat motifs\r\nwere the most common (37.6%) followed by GA/CT repeat motifs (25.9%). The primer pairs could be\r\ndesigned for a total of 170 SSRs and were optimized initially on two genotypes. 104 (61.2%) primer pairs\r\nyielded scorable amplicon and 46 (44.2%) primers showed polymorphism among 32 cultivated groundnut\r\ngenotypes. The polymorphic SSR markers detected 2 to 5 alleles with an average of 2.44 per locus. The\r\npolymorphic information content (PIC) value for these markers varied from 0.12 to 0.75 with an average\r\nof 0.46. Based on 112 alleles obtained by 46 markers, a phenogram was constructed to understand the\r\nrelationships among the 32 genotypes. Majority of the genotypes representing subspecies hypogaea were\r\ngrouped together in one cluster, while the genotypes belonging to subspecies fastigiata were grouped\r\nmainly under two clusters.\r\nConclusion: Newly developed set of 104 markers extends the repertoire of SSR markers for cultivated\r\ngroundnut. These markers showed a good level of PIC value in cultivated germplasm and therefore would\r\nbe very useful for germplasm analysis, linkage mapping, diversity studies and phylogenetic relationships in\r\ncultivated groundnut as well as related Arachis species."^^ . "2008" . . "8" . "55" . . "BioMed Central"^^ . . . "BMC Plant Biology"^^ . . . . . . . . . . . . . . . . . . . . . . . "R K"^^ . "Varshney"^^ . "R K Varshney"^^ . . "T D"^^ . "Long"^^ . "T D Long"^^ . . "L M"^^ . "Cuc"^^ . "L M Cuc"^^ . . "E S"^^ . "Mace"^^ . "E S Mace"^^ . . "J H"^^ . "Crouch"^^ . "J H Crouch"^^ . . "V D"^^ . "Quang"^^ . "V D Quang"^^ . . . . . . "Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea) (PDF)"^^ . . . . . . . . . "Isolation_and_characterization..groundnut.pdf"^^ . . . "Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea) (Image (JPEG))"^^ . . . . . . "preview.jpg"^^ . . . "Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea) (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #2696 \n\nIsolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)\n\n" . "text/html" . . . "Groundnut"@en . .