"2696","13","archive","14",,,"disk0/00/00/26/96","2011-10-27 14:24:40","2011-10-27 14:24:40","2011-10-27 14:24:40","article",,,"show","Library-ICRISAT@cgiar.org",,,"","","","","","","","","","","0",,,"Cuc","L M","","","","","","","Cuc","L M","","","","",,,,,"","",,,,,"","","ICRISAT(Patancheru)","India","Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea)","pub","s1.3","","","public",,,,,,"Background: Cultivated peanut or groundnut (Arachis hypogaea L.) is the fourth most important oilseed
crop in the world, grown mainly in tropical, subtropical and warm temperate climates. Due to its origin
through a single and recent polyploidization event, followed by successive selection during breeding
efforts, cultivated groundnut has a limited genetic background. In such species, microsatellite or simple
sequence repeat (SSR) markers are very informative and useful for breeding applications. The low level of
polymorphism in cultivated germplasm, however, warrants a need of larger number of polymorphic
microsatellite markers for cultivated groundnut.
Results: A microsatellite-enriched library was constructed from the genotype TMV2. Sequencing of 720
putative SSR-positive clones from a total of 3,072 provided 490 SSRs. 71.2% of these SSRs were perfect
type, 13.1% were imperfect and 15.7% were compound. Among these SSRs, the GT/CA repeat motifs
were the most common (37.6%) followed by GA/CT repeat motifs (25.9%). The primer pairs could be
designed for a total of 170 SSRs and were optimized initially on two genotypes. 104 (61.2%) primer pairs
yielded scorable amplicon and 46 (44.2%) primers showed polymorphism among 32 cultivated groundnut
genotypes. The polymorphic SSR markers detected 2 to 5 alleles with an average of 2.44 per locus. The
polymorphic information content (PIC) value for these markers varied from 0.12 to 0.75 with an average
of 0.46. Based on 112 alleles obtained by 46 markers, a phenogram was constructed to understand the
relationships among the 32 genotypes. Majority of the genotypes representing subspecies hypogaea were
grouped together in one cluster, while the genotypes belonging to subspecies fastigiata were grouped
mainly under two clusters.
Conclusion: Newly developed set of 104 markers extends the repertoire of SSR markers for cultivated
groundnut. These markers showed a good level of PIC value in cultivated germplasm and therefore would
be very useful for germplasm analysis, linkage mapping, diversity studies and phylogenetic relationships in
cultivated groundnut as well as related Arachis species.","2008",,,"BMC Plant Biology","8","55","BioMed Central",,"1-11",,,,,,,,,,,"TRUE",,,,,,,,"","http://dx.doi.org/10.1186/1471-2229-8-55","http://scholar.google.co.in/scholar?as_q=Isolation+and+characterization+of+novel+microsatellite+markers+and+their+application+for+diversity+assessment+in+cultivated+groundnut+%28Arachis+hypogaea%29&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=","author",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"2696",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Mace","E S","","",,,,,"Mace","E S","","",,,,,,,,,,,,,,,"Vietnamese Academy Agriculture Science","Vietnam",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"2696",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Crouch","J H","","",,,,,"Crouch","J H","","",,,,,,,,,,,,,,,"Hermitage Research Station","Australia",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"2696",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Quang","V D","","",,,,,"Varshney","R K","","",,,,,,,,,,,,,,,"CIMMYT(Mexico)","Mexico",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"2696",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Long","T D","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"2696",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Varshney","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,