eprintid: 2602
rev_number: 7
eprint_status: archive
userid: 30
dir: disk0/00/00/26/02
datestamp: 2011-10-15 08:01:56
lastmod: 2011-10-15 08:01:56
status_changed: 2011-10-15 08:01:56
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Sujata, V
creators_name: Sivaramakrishnan, S
creators_name: Rai, K N
creators_name: Seetha, K
icrisatcreators_name: Sujata, V
icrisatcreators_name: Sivaramakrishnan, S
icrisatcreators_name: Rai, K N
icrisatcreators_name: Seetha, K
affiliation: ICRISAT(Patancheru)
country: India
title: A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA.
ispublished: pub
subjects: S1.5
full_text_status: restricted
note: We thank Mr.R.Luke for technical assistance.
abstract: A new source of cytoplasmic male sterility (cms) in pearl millet (Pennisetum glaucum (L.) R.Br.) derived from a half-sib progeny of the Early Gene Pool (EGP 261) and used in a male-sterile line, ICMA 90111, was compared with other known cms sources for RFLP of mitochondrial (mt) DNA. Southern blot hybridization of mtDNA from ICMA 90111 digested with several restriction enzymes and probed with homologous mtDNA clones from pearl millet and heterologous gene clones from maize and wheat revealed the RFLP patterns of ICMA 90111 distinct from others studied so far. The dendrogram of male-sterile lines constructed from the Southern blot hybridization patterns indicated that ICMA 90111 represents a separate group. Our results suggest that this source of cms is unique in several respects.Key words: Pennisetum glaucum, cytoplasmic male sterility, mitochondrial DNA, RFLP.
date: 1994
date_type: published
publication: Genome
volume: 37
number: 3
publisher: NRC Research Press
pagerange: 482-486
refereed: TRUE
issn: 0831-2796
official_url: http://dx.doi.org/10.1139/g94-067
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22+++++A+new+source+of+cytoplasmic+male+sterility+in+pearl+millet%3A+RFLP+analysis+of+mitochondrial+DNA.%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
citation:   Sujata, V and Sivaramakrishnan, S and Rai, K N and Seetha, K  (1994) A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA.  Genome, 37 (3).  pp. 482-486.  ISSN 0831-2796     
document_url: http://oar.icrisat.org/2602/1/A_new_source_of_cytoplasmic_male_sterility_in_pearl_millet_RFLP_analysis_of_mitochondrial_DNA.pdf