eprintid: 2341
rev_number: 8
eprint_status: archive
userid: 6
dir: disk0/00/00/23/41
datestamp: 2011-10-12 08:31:33
lastmod: 2011-10-12 08:31:33
status_changed: 2011-10-12 08:31:33
type: thesis
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Reddy, Y C
icrisatcreators_name: Sharma, H C
supervisors_name: Venkateswarlu, G
supervisors_name: Sharma, H C
supervisors_id: OU
supervisors_id: ICRISAT
affiliation: Osmania University(Hyderabad)
country: India
title: Studies on Bacillus thuringiensis proteases involved in the production of insecticidal toxins from protoxins
ispublished: submitted
subjects: s2.7
full_text_status: public
abstract: Two mutants of Btk HD1 were isolated, one having elevated protease activity
(Btk-e, hyper producing) and the other with a deficiency (Btk-q). These
mutants were characterized by X-ray film spot assay, gelatin ymography,
azocasein analysis, western blot analysis, plasmid profiles, PCR, and Southern
blot analysis. Btkq mutant displayed 2-3 fold higher activity against
Helieoverpa armigera, Spodoptera litura and Chilo partellus, whereas Btk-e
mutant displayed no significant enhancement in activity, but slightly lower
activity against C. partellus. Protease deficient mutant Btk-q did not reveal the
presence of any 66 kDa toxin band as observed with the wild type strain Btk
HD1. It showed only 132 kda protoxin. The protoxins isolated from Btk HDI...
date: 2001
date_type: submitted
institution: Osmania University
department: Department of Biochemistry
thesis_type: phd
related_url_url: http://scholar.google.co.in/scholar?as_q=Studies+on+Bacillus+thuringiensis+proteases+involved+in+the+production+of+insecticidal+toxins+from+protoxins&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yh
related_url_type: author
funders: Government of India - Department of Biotechnology 
citation:   Reddy, Y C  (2001) Studies on Bacillus thuringiensis proteases involved in the production of insecticidal toxins from protoxins.  PHD thesis, Osmania University.   
document_url: http://oar.icrisat.org/2341/1/62968.pdf