<mods:mods version="3.3" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mods:titleInfo><mods:title>Studies on Bacillus thuringiensis proteases involved in the production of insecticidal toxins from protoxins</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">Y C</mods:namePart><mods:namePart type="family">Reddy</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Two mutants of Btk HD1 were isolated, one having elevated protease activity&#13;
(Btk-e, hyper producing) and the other with a deficiency (Btk-q). These&#13;
mutants were characterized by X-ray film spot assay, gelatin ymography,&#13;
azocasein analysis, western blot analysis, plasmid profiles, PCR, and Southern&#13;
blot analysis. Btkq mutant displayed 2-3 fold higher activity against&#13;
Helieoverpa armigera, Spodoptera litura and Chilo partellus, whereas Btk-e&#13;
mutant displayed no significant enhancement in activity, but slightly lower&#13;
activity against C. partellus. Protease deficient mutant Btk-q did not reveal the&#13;
presence of any 66 kDa toxin band as observed with the wild type strain Btk&#13;
HD1. It showed only 132 kda protoxin. The protoxins isolated from Btk HDI...</mods:abstract><mods:classification authority="lcc">Entomology</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2001</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Osmania University;Department of Biochemistry</mods:publisher></mods:originInfo><mods:genre>Thesis</mods:genre></mods:mods>