OAR@ICRISATInduction of Androgenesis as a Consequence of Wide crossing in chickpea.NMallikarjunaauthorD RJadhavauthorHClarkeauthorCCoyneauthorF JMuehlbauerauthorAndrogenesis was observed in a wide cross of Cicer arietinum × C. pinnatifidum. Hybrids between C. arietinum × C. pinnatifidum were obtained after rescuing the hybrid embryos in vitro. The hybrids were initially devoid of any chlorophyll pigment and were albinos. Upon continuous culture in a zeatin-rich medium and in the presence of light, the hybrids turned semi-green. Hybrid shoots were grafted to chickpea rootstocks to obtain hybrid plants. None of the hybrid plants flowered. When the nutrient solution with zeatin (1.0 mg/litre) was added, flower buds were observed on hybrid plants. Flower buds were fragile, albino to semi-green, but with normal morphology. Anthers were squashed in acetocarmine and divisions were observed in some of the microspores. The number of divisions varied from 4 to 6. Adding nutrient solution with zeatin (1.0 mg/litre) to in vivo-grown chickpea plants did not induce division in the microspores. Sixteen hybrid plants were obtained. The number of microspores/pollen grains in an anther varied from 11 to 151, compared to more than 500 pollen grains in cultivated chickpea. The number of pollen grains that had undergone microsporogenesis and induction of androgenesis varied from plant to plant. Percent androgenic pollen grains varied from 0 to 100%. Plant nos. 8, 11 and 12 did not have any androgenic pollen grains, whereas in plant nos. 14 and 16, all the pollen grains were androgenic or had multicellular microspores. The number of cells in multicellular microspores in plant nos. 14 and 16 varied from 8 to 10 unlike 4-6 cells in multicellular microspores in other hybrid plants that had androgenic microspores. This is the first report of the production of multicellular microspores as a result of wide crossing.Chickpea2005International Crops Research Institute for the Semi-Arid Tropics Article

For work being deposited by its own author: In self-archiving this collection of files and associated bibliographic metadata, I grant OAR@ICRISAT the right to store them and to make them permanently available publicly for free on-line. I declare that this material is my own intellectual property and I understand that OAR@ICRISAT does not assume any responsibility if there is any breach of copyright in distributing these files or metadata. (All authors are urged to prominently assert their copyright on the title page of their work.)

For work being deposited by someone other than its author: I hereby declare that the collection of files and associated bibliographic metadata that I am archiving at OAR@ICRISAT) is in the public domain. If this is not the case, I accept full responsibility for any breach of copyright that distributing these files or metadata may entail.

Clicking on the deposit button indicates your agreement to these terms.