eprintid: 2137
rev_number: 11
eprint_status: archive
userid: 6
dir: disk0/00/00/21/37
datestamp: 2011-10-10 06:11:06
lastmod: 2011-10-10 06:12:02
status_changed: 2011-10-10 06:11:06
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Devi, K T
creators_name: Mayo, M A
creators_name: Reddy, K L N
creators_name: Delfosse, P
creators_name: Reddy, G
creators_name: Reddy, S V
creators_name: Reddy, D V R
icrisatcreators_name: Devi, K T
icrisatcreators_name: Delfosse, P
icrisatcreators_name: Reddy, S V
icrisatcreators_name: Reddy, D V R
affiliation: ICRISAT(Patancheru)
affiliation: Scottish Crop Research Institute(Dundee)
affiliation: Osmania University(Hyderabad)
country: India
country: UK
title: Production and characterization of monoclonal antibodies
for aflatoxin B1
ispublished: pub
subjects: s2.4
full_text_status: public
note: The financial support of the UK Department For International
Development ([DFID] project number R7083) is
gratefully acknowledged. We are grateful to Dr Anjali
Karande, Department of Biochemistry, Indian Institute of
Sciences, Bangalore, for her advice in the production of
monoclonal antibodies. M. Mayo is supported by the Scottish
Office Agriculture, Environment and Fisheries Department,
UK. This paper was submitted as journal article number
2323 by ICRISAT.
abstract: Hybridomas that secreted antibodies for aflatoxin B1 were selected using
two immunization protocols referred to as A and B. Protocol A is a standard
immunization method and resulted in the selection of only two clones that produced
monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization
schedule which resulted in the generation of 10 hybridomas is described. Of the
10, one antibody was highly specific to B1, four antibodies reacted equally strongly
with B1, G1 and weakly with B2. Another four reacted strongly with B1 and
weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2.
Interestingly all the 10 antibodies showed little or no cross-reaction with G2.
date: 1999
date_type: published
publication: Letters In Applied Microbiology
volume: 29
number: 5
publisher: Blackwell Publishing
pagerange: 284-288
id_number: JA 2323
refereed: TRUE
official_url: http://dx.doi.org/10.1046/j.1472-765X.1999.00685.x
related_url_url: http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.1999.00685.x/pdf
related_url_url: http://scholar.google.co.in/scholar?as_q=Production+and+characterization+of+monoclonal+antibodies+for+aflatoxin+B1&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_sdtp=on&as_sdtf=&as
related_url_type: pub
related_url_type: author
funders: Department for International Development(DFID)
citation:   Devi, K T and Mayo, M A and Reddy, K L N and Delfosse, P and Reddy, G and Reddy, S V and Reddy, D V R  (1999) Production and characterization of monoclonal antibodies for aflatoxin B1.  Letters In Applied Microbiology, 29 (5).  pp. 284-288.      
document_url: http://oar.icrisat.org/2137/1/LettersInAppMicr29_2_284-288_1999.pdf