eprintid: 2086
rev_number: 14
eprint_status: archive
userid: 30
dir: disk0/00/00/20/86
datestamp: 2011-10-08 12:20:26
lastmod: 2012-01-05 06:04:35
status_changed: 2011-10-08 12:20:26
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Girijashankar, V
creators_name: Sharma, H C
creators_name: Sharma, K K
creators_name: Swathisree, V
creators_name: Prasad, L S
creators_name: Bhat, B V
creators_name: Royer, M
creators_name: Secundo, B S
creators_name: Narasu, M L
creators_name: Altosaar, I
creators_name: Seetharama, N
icrisatcreators_name: Girijashankar, V
icrisatcreators_name: Sharma, H C
icrisatcreators_name: Sharma, K K
icrisatcreators_name: Prasad, L S
icrisatcreators_name: Bhat, B V
icrisatcreators_name: Seetharama, N
affiliation: ICRISAT(Patancheru)
affiliation: Jawaharlal Nehru Technological University(Hyderabad)
affiliation: CIRAD(Montpeillar) 
affiliation: IBMB(Barcelona)
affiliation: Roger-Guindon Hall(Ottawa)
country: India
country: France
country: Spain
country: Canada
title: Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus) 
ispublished: pub
subjects: s1.4
full_text_status: public
keywords: Chilo partellus, cry1Ac, Insect resistance, Sorghum bicolor,  Wound-inducible promoter

note: This work was supported by AGROPOLIS
 Advanced Research platform, France, for which the authors are
  thankful. V. Girijashankar is grateful to University Grants Commission (UGC), New Delhi, India for providing a research fellowship.

abstract: Transgenic sorghum plants expressing a synthetic cry1Ac gene from Bacillus thuringiensis (Bt) under the control of a wound-inducible promoter from the maize protease inhibitor gene (mpiC1) were produced via particle bombardment of shoot apices. Plants were regenerated from the transformed shoot apices via direct somatic embryogenesis with an intermittent three-step selection strategy using the herbicide Basta. Molecular characterisation based on polymerase chain reaction and Southern blot analysis revealed multiple insertions of the cry1Ac gene in five plants from three independent transformation events. Inheritance and expression of the Bt gene was confirmed in T1 plants. Enzyme-linked immunosorbant assay indicated that Cry1Ac protein accumulated at levels of 1–8 ng per gram of fresh tissue in leaves that were mechanically wounded. Transgenic sorghum plants were evaluated for resistance against the spotted stem borer (Chilo partellus Swinhoe) in insect bioassays, which indicated partial resistance to damage by the neonate larvae of the spotted stem borer. Reduction in leaf damage 5 days after infestation was up to 60%; larval mortality was 40%, with the surviving larvae showing a 36% reduction in weight over those fed on control plants. Despite the low levels of expression of Bt delta-endotoxin under the control of the wound-inducible promoter, the transgenic plants showed partial tolerance against first instar larvae of the spotted stem borer.
date: 2005
date_type: published
publication: Plant Cell Reports
volume: 24
number: 9
publisher: Springer Verlag
pagerange: 513-522
refereed: TRUE
issn: 0721-7714
official_url: http://dx.doi.org/10.1007/s00299-005-0947-7
related_url_url: http://scholar.google.co.in/scholar?hl=en&q=allintitle%3A+%22+Development+of+transgenic+sorghum+for+insect+resistance+against+the+spotted+stem+borer+(Chilo+partellus)%22&btnG=Search&as_sdt=0%2C5&as_ylo=&as_vis=0
related_url_type: author
citation:   Girijashankar, V and Sharma, H C and Sharma, K K and Swathisree, V and Prasad, L S and Bhat, B V and Royer, M and Secundo, B S and Narasu, M L and Altosaar, I and Seetharama, N  (2005) Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus).  Plant Cell Reports, 24 (9).  pp. 513-522.  ISSN 0721-7714     
document_url: http://oar.icrisat.org/2086/1/Development_of_transgenic_sorghum_for_insect_resistance_against_the_spotted_stem_borer_%28Chilo_partellus%29.pdf