eprintid: 1996
rev_number: 8
eprint_status: archive
userid: 19
dir: disk0/00/00/19/96
datestamp: 2011-10-07 05:41:58
lastmod: 2011-10-07 05:41:58
status_changed: 2011-10-07 05:41:58
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
item_issues_count: 0
creators_name: Subramanian, V
creators_name: Gurtu, S
creators_name: Rao, R C N
creators_name: Nigam, S N
icrisatcreators_name: Gurtu, S
icrisatcreators_name: Rao, R C N
icrisatcreators_name: Nigam, S N
affiliation: MAHYCO(Kallakal)
affiliation: ICRISAT(Patancheru)
country: India
title: Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay
ispublished: pub
subjects: s1.3
full_text_status: public
keywords: Arachis hypogaea L., RAPD, DNA polymorphism, Oligonucleotide, random primers.
abstract: Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement programme. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing a RAPD assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits
date: 2000
date_type: published
publication: Genome
volume: 43
number: 4
publisher: NRC Press
pagerange: 656-660
refereed: TRUE
official_url: http://www.nrcresearchpress.com/doi/pdf/10.1139/g00-034
related_url_url: http://scholar.google.co.in/scholar?q=Identification+of+DNA+polymorphism+in+cultivated&hl=en&btnG=Search&as_sdt=1%2C5&as_sdtp=on
related_url_type: author
citation:   Subramanian, V and Gurtu, S and Rao, R C N and Nigam, S N  (2000) Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay.  Genome, 43 (4).  pp. 656-660.      
document_url: http://oar.icrisat.org/1996/1/Genome_43_4_656-660_2000.pdf