eprintid: 1921
rev_number: 11
eprint_status: archive
userid: 14
dir: disk0/00/00/19/21
datestamp: 2011-09-26 04:15:01
lastmod: 2011-09-26 04:15:01
status_changed: 2011-09-26 04:15:01
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Sharma, K K
creators_name: Lavanya, M
creators_name: Anjaiah, V
icrisatcreators_name: Sharma, K K
icrisatcreators_name: Lavanya, M
icrisatcreators_name: Anjaiah, V
affiliation: ICRISAT(Patancheru)
country: India
title: A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications
ispublished: pub
subjects: s1.3
full_text_status: restricted
keywords: DNA isolation, PCR amplification, peanut, Southern hybridization, transgenic plants
note: We thank Dr. Rodomiro Ortiz for his encouragement and suggestions on the
manuscript. Part of the funding for this work was provided by grants from OPEC
and Asian Development Bank.
abstract: Numerous methods are available for isolation of plant genomic DNA, but in
practice these procedures are empirical due to variability in plant tissue composition. Consistent
isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic
due to the presence of phenolic compounds and polysaccharides. Inconsistencies
in extraction results can be attributed to the age and growth stage of the plant material analyzed.
Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides
that can contaminate DNA during isolation. We show that four published protocols could
not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern
hybridization. We have devised a new protocol that uses DEAE-cellulose purification to
isolate peanut DNA useful for downstream applications.
date: 2000
date_type: published
publication: Plant Molecular Biology Reporter
volume: 18
number: 4
publisher: Springer
pagerange: 393a-393h
refereed: TRUE
issn: 1572-9818
official_url: http://dx.doi.org/10.1007/BF02825068
related_url_url: http://scholar.google.co.in/scholar?as_q=A+Method+for+Isolation+and+Purification+of+Peanut+Genomic+DNA+Suitable+for+Analytical+Applications&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=
related_url_type: author
funders: OPEC
funders: Asian Development Bank
citation:   Sharma, K K and Lavanya, M and Anjaiah, V  (2000) A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications.  Plant Molecular Biology Reporter, 18 (4).  393a-393h.  ISSN 1572-9818     
document_url: http://oar.icrisat.org/1921/1/PlMolBioRep18_4_393a-309h_2000.pdf