TY  - JOUR
AV  - restricted
A1  - Sharma, K K
A1  - Lavanya, M
A1  - Anjaiah, V
TI  - A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications
UR  - http://dx.doi.org/10.1007/BF02825068
JF  - Plant Molecular Biology Reporter
SN  - 1572-9818
PB  - Springer
N1  - We thank Dr. Rodomiro Ortiz for his encouragement and suggestions on the
manuscript. Part of the funding for this work was provided by grants from OPEC
and Asian Development Bank.
N2  - Numerous methods are available for isolation of plant genomic DNA, but in
practice these procedures are empirical due to variability in plant tissue composition. Consistent
isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic
due to the presence of phenolic compounds and polysaccharides. Inconsistencies
in extraction results can be attributed to the age and growth stage of the plant material analyzed.
Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides
that can contaminate DNA during isolation. We show that four published protocols could
not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern
hybridization. We have devised a new protocol that uses DEAE-cellulose purification to
isolate peanut DNA useful for downstream applications.
KW  - DNA isolation
KW  -  PCR amplification
KW  -  peanut
KW  -  Southern hybridization
KW  -  transgenic plants
Y1  - 2000///
SP  - 393a
ID  - icrisat1921
EP  - 393h
VL  - 18
IS  - 4
ER  -