<mods:mods version="3.3" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mods:titleInfo><mods:title>A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">K K</mods:namePart><mods:namePart type="family">Sharma</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Lavanya</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">V</mods:namePart><mods:namePart type="family">Anjaiah</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>Numerous methods are available for isolation of plant genomic DNA, but in&#13;
practice these procedures are empirical due to variability in plant tissue composition. Consistent&#13;
isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic&#13;
due to the presence of phenolic compounds and polysaccharides. Inconsistencies&#13;
in extraction results can be attributed to the age and growth stage of the plant material analyzed.&#13;
Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides&#13;
that can contaminate DNA during isolation. We show that four published protocols could&#13;
not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern&#13;
hybridization. We have devised a new protocol that uses DEAE-cellulose purification to&#13;
isolate peanut DNA useful for downstream applications.</mods:abstract><mods:classification authority="lcc">Groundnut</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2000</mods:dateIssued></mods:originInfo><mods:originInfo><mods:publisher>Springer</mods:publisher></mods:originInfo><mods:genre>Article</mods:genre></mods:mods>