%0 Journal Article
%@ 1572-9818
%A Sharma, K K
%A Lavanya, M
%A Anjaiah, V
%D 2000
%F icrisat:1921
%I Springer
%J Plant Molecular Biology Reporter
%K DNA isolation, PCR amplification, peanut, Southern hybridization, transgenic plants
%N 4
%P 393a-393h
%T A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications
%U http://oar.icrisat.org/1921/
%V 18
%X Numerous methods are available for isolation of plant genomic DNA, but in  practice these procedures are empirical due to variability in plant tissue composition. Consistent  isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic  due to the presence of phenolic compounds and polysaccharides. Inconsistencies  in extraction results can be attributed to the age and growth stage of the plant material analyzed.  Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides  that can contaminate DNA during isolation. We show that four published protocols could  not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern  hybridization. We have devised a new protocol that uses DEAE-cellulose purification to  isolate peanut DNA useful for downstream applications.
%Z We thank Dr. Rodomiro Ortiz for his encouragement and suggestions on the  manuscript. Part of the funding for this work was provided by grants from OPEC  and Asian Development Bank.