"1916","11","archive","14",,,"disk0/00/00/19/16","2011-09-26 06:25:02","2011-09-26 06:25:39","2011-09-26 06:25:01","article",,,"show","Library-ICRISAT@cgiar.org",,,"","","","","","","","","","","0",,,"Pazoutová","S","","","","","","","Bandyopadhyay","R","","","","",,,,,"","",,,,,"","","Institute of Microbiology Czech Academy of Sciences(Prague)","Czech Republic","Relations Among Sorghum Ergot Isolates from the Americas, Africa, India, and Australia","pub","s1.4","","","public",,,"Sphacelia sorghi, sugary disease",,"We thank P. Vízner for excellent technical assistance;
M. Ryley, S. Indira, and N. McLaren for
providing the sorghum ergot isolates; and C.
Magill and B. Fry for useful comments on an
initial draft of the manuscript.","Sorghum ergot, initially restricted to Asia and Africa, was recently found in the Americas and
Australia. Three species causing the disease have been reported: Claviceps sorghi in India, C.
sorghicola in Japan, and C. africana in all ergot-positive countries. The objective of our study
was to study the intraspecific variation in C. africana isolates in the Americas, Africa, India,
and Australia. We confirmed C. africana, C. sorghi, and C. sorghicola as different species using
differences in nucleotide sequences of internal transcribed spacer 1 and 5.8S rDNA regions.
Sequences of this region obtained from the representative American, Indian, and Australian
isolates of C. africana were identical. In addition, random amplified polymorphic DNA (RAPD)
banding patterns of sorghum ergot pathogen isolates from the United States, Mexico, Puerto
Rico, Bolivia, Australia, and India were evaluated with nearly 100 primers. A total of 65 primers
gave identical patterns for all isolates, which confirmed that all were C. africana. The identity
of RAPD pattern and rDNA sequence of Indian isolates with those of C. africana confirmed
that the species is now present in India. Only 20 primers gave small pattern differences and 7 of
them were used for routine testing. All of the American isolates were identical and three isolates
of the same type were also found in South Africa, suggesting Africa as the origin of the
invasion clone in the Americas. Australian and Indian isolates were distinguishable by a single
band difference; therefore, migration from the Asian region to Australia is suspected. Another
distinct group was found in Africa. Cluster analysis of the informative bands revealed that the
American and African group are on the same moderately (69%) supported clade. Isolates from
Australia and India belonged to another clade.","2000","published",,"Plant Disease","84","4","American Phytopathological Society",,"437-442",,,,,,,,,,,"TRUE",,"0191-2917",,,,,,"","http://dx.doi.org/10.1094/PDIS.2000.84.4.437","http://scholar.google.co.in/scholar?as_q=Relations+Among+Sorghum+Ergot+Isolates+from+the+Americas%2C+Africa%2C+India%2C+and+Australia&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yhi=&as_sdt=1.&as_","author",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"1916",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bandyopadhyay","R","","",,,,,,,,,,,,,,,,,,,,,,,"ICRISAT(Patancheru)","India",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2000.84.4.437","pub",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"1916",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Frederickson","D E","","",,,,,,,,,,,,,,,,,,,,,,,"Matopos Research Statio(Bulawayo)","Zimbabwe",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"1916",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Mantle","P G","","",,,,,,,,,,,,,,,,,,,,,,,"Imperial College of Science(London)","UK",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"1916",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Frederiksen","R A","","",,,,,,,,,,,,,,,,,,,,,,,"Texas A & M University(College Station)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,