eprintid: 1891
rev_number: 11
eprint_status: archive
userid: 14
dir: disk0/00/00/18/91
datestamp: 2011-09-23 09:33:13
lastmod: 2011-09-23 09:33:13
status_changed: 2011-09-23 09:33:13
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Bragard, C
creators_name: Duncan, G H
creators_name: Wesley, S V
creators_name: Naidu, R A
creators_name: Mayo, M A
icrisatcreators_name: Wesley, S V
icrisatcreators_name: Naidu, R A
affiliation: Scottish Crop Research Institute(Dundee)
affiliation: Universite Catholique de Louvain(Louvain-la-Neuve)
affiliation: ICRISAT(Patancheru)
country: UK
country: Belgium
country: India
title: Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus
ispublished: pub
subjects: s1.3
full_text_status: restricted
abstract: cDNA copies of the coat protein (CP) gene of Indian peanut clump virus (IPCV)-H were introduced into cells of Nicotiana benthamiana or Escherichia coli by transformation with vectors based on pROKII or pET respectively. In both plant and bacterial cells, IPCV CP was expressed and assembled to form virus-like particles (VLP). In plant extracts, the smallest preponderant particle length was c. 50 nm. Other abundant lengths were c. 85 and c. 120 nm. The commonest VLP length in bacterial extracts was c. 30 nm. Many of the longer VLP appeared to comprise aggregates of shorter particles. The lengths of the supposed 'monomer' VLP corresponded approximately to those expected for encapsidated CP gene transcript RNA. Immunocapture RT-PCR, using primers designed to amplify the CP gene, confirmed that the VLP contained RNA encoding IPCV-H CP. The results show that encapsidation does not require the presence of the 5′-terminal untranslated sequence of the virus RNA and suggest that if there is an 'origin of assembly' motif or sequence, it lies within the CP gene. When transgenic plants expressing IPCV-H CP were inoculated with IPCV-L, a strain that is serologically distinct from IPCV-H, the virus particles that accumulated contained both types of CP.
date: 2000
date_type: published
publication: Journal of General Virology
volume: 81
number: 1
publisher: Society for General Microbiology
pagerange: 267-272
refereed: TRUE
issn: 0022-1317
official_url: http://vir.sgmjournals.org/content/81/1/267.full.pdf+html
related_url_url: http://scholar.google.co.in/scholar?as_q=Virus-like+particles+assemble+in+plants+and+bacteria+expressing+the+coat+protein+gene+of+Indian+peanut+clump+virus&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=
related_url_type: author
citation:   Bragard, C and Duncan, G H and Wesley, S V and Naidu, R A and Mayo, M A  (2000) Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus.  Journal of General Virology, 81 (1).  pp. 267-272.  ISSN 0022-1317     
document_url: http://oar.icrisat.org/1891/1/JGenVirol81%281%29267-272.pdf