http://oar.icrisat.org/id/eprint/187 2011-08-16T06:39:40Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/187/ Chickpea Hybridization Using In Vitro Techniques Mallikarjuna, N Muehlbauer, F J Chickpea Tissue culture techniques play an important role in the utilization of wild Cicer species for the improvement of cultivated chickpea. Utilization of wild Cicer species has become essential as a series of evolutionary bottlenecks have narrowed the genetic base of chickpea, thus making it susceptible to a range of diseases and pests. Crosses with wild Cicer can broaden its genetic base and introduce useful traits. Except for two wild species, none of the other Cicer species are cross-compatible. To use a range of Cicer species for the improvement of chickpea, embryo rescue and tissue culture techniques are necessary. The success of the cross with incompatible species depended on a range of techniques including the application of growth regulators to pollinated pistils and saving aborting embryos in vitro. Further, the chances of successful transfer of hybrid shoots to soil are greater if the hybrid shoots are grafted to chickpea stocks Springer 2011 Book Section PeerReviewed application/pdf en http://oar.icrisat.org/187/1/nalini1.pdf Mallikarjuna, N and Muehlbauer, F J (2011) Chickpea Hybridization Using In Vitro Techniques. In: Plant Embryo Culture: Methods and Protocols. Methods in Molecular Biology, vol. 710 . Springer, Germany, pp. 93-105. ISBN 1617379875 http://dx.doi.org/10.1007/978-1-61737-988-8_8 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage