TY - JOUR AV - restricted A1 - Naidu, R A A1 - Miller, J S A1 - Mayo, M A A1 - Wesley, S V A1 - Reddy, A S TI - The nucleotide sequence of Indian peanut clump virus RNA 2: sequence comparisons among pecluviruses UR - http://dx.doi.org/10.1007/s007050070061 JF - Archives of Virology SN - 1432-8798 PB - Springer N1 - R. A. Naidu is grateful to the Underwood Fund administered by the Biotechnology and Biological Sciences Research Council, UK, for financial assistance to carry out part of this research. J. S. Millerwas funded by theUKOverseas Development Administration (currently the Department for International Development) (project R5417) and M. A. Mayo was supported by the Scottish Office Agriculture, Environment and Fisheries Department (currently the Scottish Executive Rural Affairs Department). N2 - The RNA-2 molecule of an isolate of the L serotype of Indian peanut clump virus (IPCV) was shown to consist of 4290 nucleotides with five open reading frames (ORF). The arrangement of the ORFs resembled that in RNA-2 of peanut clump virus (PCV) from West Africa. The proteins encoded by the ORFs in IPCV-L RNA are between 32% and 93% identical to those encoded by PCV RNA. Partial sequence data for the RNA-2 of isolates of the H and T serotypes of IPCV show that the coat and P40 proteins encoded by the 5?-most ORFs of RNA-2 of IPCV-L, IPCV-H and IPCV-T are as similar to each other as any is to the corresponding proteins of PCV. A conserved motif 'F-E-x6-W' is present near the C-termini of the coat proteins of all three IPCV serotypes and of PCV, as it is in the coat proteins of other viruses that have rod-shaped particles, such as tobacco mosaic virus and tobacco rattle virus. The results support the distinction of IPCV and PCV as separate virus species, but also raise the question of how the serotypes of IPCV should be classified. Y1 - 2000/// SP - 1857 ID - icrisat1853 EP - 1866 VL - 145 IS - 9 ER -