eprintid: 1824
rev_number: 8
eprint_status: archive
userid: 14
dir: disk0/00/00/18/24
datestamp: 2011-09-21 10:19:24
lastmod: 2011-09-21 10:19:24
status_changed: 2011-09-21 10:19:24
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@cgiar.org
item_issues_count: 0
creators_name: Mythili, P K
creators_name: Seetharama, N
creators_name: Reddy, V D
icrisatcreators_name: Mythili, P K
icrisatcreators_name: Seetharama, N
affiliation: ICRISAT(Patancheru)
affiliation: Osmania University(Hyderabad)
country: India
title: Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)
ispublished: pub
subjects: s1.4
full_text_status: restricted
abstract: A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma.
date: 1999
publication: Plant Cell Reports
volume: 18
number: 5
publisher: Springer Verlag
pagerange: 424-428
refereed: TRUE
official_url: http://dx.doi.org/10.1007/s002990050597
related_url_url: http://scholar.google.co.in/scholar?as_q=Plant+regeneration+from+embryogenic+cell+suspension+cultures+of+wild+sorghum+%28Sorghum+dimidiatum+Stapf.%29&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publication=&as_ylo=&as_yh
related_url_type: author
citation:   Mythili, P K and Seetharama, N and Reddy, V D  (1999) Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.).  Plant Cell Reports, 18 (5).  pp. 424-428.      
document_url: http://oar.icrisat.org/1824/1/PlCellRep18%28424-428_1999.pdf