TY  - JOUR
N2  - A simple and efficient protocol is described for regeneration of wild sorghum (Sorghum dimidiatum) from cell suspension cultures. Fast-growing cell suspensions were established from shoot-meristem-derived callus. Plating of the suspension on Murashige and Skoog agar medium supplemented with 2.5 mg 1-1 2,4-dichlorophen-oxyacetic acid (2,4-D) resulted in the formation of embryogenic calli. High-frequency (80%) somatic embryogenesis from small cell clusters (300-400 micrometer) was observed when the cultures were initially maintained in liquid medium with reduced levels of 2,4-D (0.25 mg l-1), followed by transfer to regeneration medium. Direct plating of these small clusters on regeneration medium or transfer to liquid regeneration medium containing kinetin and 6-benzylaminopurine resulted in the development of mature somatic embryos and plantlets. The regenerants developed to maturity and were all phenotypically and cytologically norma.
AV  - restricted
A1  - Mythili, P K
A1  - Seetharama, N
A1  - Reddy, V D
TI  - Plant regeneration from embryogenic cell suspension cultures of wild sorghum (Sorghum dimidiatum Stapf.)
UR  - http://dx.doi.org/10.1007/s002990050597
JF  - Plant Cell Reports
SP  - 424
Y1  - 1999///
ID  - icrisat1824
EP  - 428
VL  - 18
PB  - Springer Verlag
IS  - 5
ER  -